PMID- 12041663
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20191025
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 75
IP  - 4
DP  - 2002 May
TI  - Cytokines regulate development of human mast cells from hematopoietic 
      progenitors.
PG  - 350-6
AB  - Combination of stem cell factor (SCF) and interleukin-6 (IL-6) significantly 
      promoted proliferation of human mast cells from cord blood CD34+ cells. Most of 
      the cells, cultured in the presence of SCF and IL-6 for 10 weeks, expressed c-kit 
      and contained a significant amount of histamine and tryptase and a low amount of 
      chymase. Both tryptase-positive chymase-negative mast cells (MC(T)) and 
      tryptase-positive chymase-positive mast cells (MC(TC)) were found in the same 
      colony derived from a single cord blood CD34+ cell, suggesting that MC(T) and 
      MC(TC) develop from common precursor cells. Single-cell culture of CD34+ cells 
      revealed that committed mast cell progenitors are included in CD34+CD38+HLA-DR- 
      cells. IL-4 significantly enhanced high-affinity immunoglobulin E (IgE) receptor 
      (FcepsilonRI) alpha-chain messenger RNA expression and induced FcepsilonRI on 
      SCF-dependent cord blood-derived human mast cells, resulting in high 
      histamine-releasing activity upon cross-linking of FcepsilonRI. Another factor 
      that up-regulated FcepsilonRI was IgE, and a combination of IL-4 and IgE markedly 
      augmented FcepsilonRI expression on the mast cells. IL-4 and IgE may enhance 
      FcepsilonRI expression by distinct mechanisms; IL-4 promotes FcepsilonRI 
      alpha-chain gene transcription and thus increases alpha-chain protein synthesis 
      in the cells, whereas the binding of IgE may anchor the FcepsilonRI on the cell 
      surface, resulting in suppression of internalization of FcepsilonRI. Mast cells 
      are progeny of hematopoietic stem cells. Recent discovery of a 
      xenotransplantation model revealed that human hematopoietic stem cells can 
      proliferate and differentiate into mature mast cells in the mouse skin 3 months 
      after transplantation of human cord blood CD34+ cells, suggesting that this model 
      may pave the way to clarification of the functions of human mast cells in vivo.
FAU - Nakahata, Tatsutoshi
AU  - Nakahata T
AD  - Department of Pediatrics, Graduate School of Medicine, Kyoto University, Japan. 
      tnakaha@kuph.kyoto-u.ac.jp
FAU - Toru, Hano
AU  - Toru H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/drug effects
MH  - Cytokines/pharmacology/*physiology
MH  - Hematopoietic Stem Cells/*cytology/drug effects
MH  - Humans
MH  - Mast Cells/*cytology
MH  - Phenotype
RF  - 92
EDAT- 2002/06/04 10:00
MHDA- 2003/07/12 05:00
CRDT- 2002/06/04 10:00
PHST- 2002/06/04 10:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2002/06/04 10:00 [entrez]
AID - 10.1007/BF02982123 [doi]
PST - ppublish
SO  - Int J Hematol. 2002 May;75(4):350-6. doi: 10.1007/BF02982123.