PMID- 12042648
OWN - NLM
STAT- MEDLINE
DCOM- 20020626
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 73
IP  - 10
DP  - 2002 May 27
TI  - Improved flow cytometric detection of donor-specific HLA class II antibodies by 
      heat inactivation.
PG  - 1611-4
AB  - Flow cytometry is a powerful technique for T-cell crossmatching but is prone to 
      false-positive reactions with B cells. In this study the flow cytometry 
      crossmatch (FCXM) was performed in 319 cases, using the patient's serum untreated 
      and incubated at 56 degrees C for 30 minutes. Heat treatment inhibited B-cell 
      reactivity in 30 of 39 cases. Flow cytometry testing with latex beads coated with 
      human leukocyte antigen (HLA) class II antigens showed no class II antibodies in 
      sera that were completely inhibited by heat treatment. There was no inhibition of 
      class I antibodies to either T or B cells, or of class II antibodies to B cells. 
      Experiments with aggregated IgG showed that inhibition by heat treatment is 
      likely due to the prevention of complement binding to aggregates or dissociation 
      of aggregates, or both. We conclude that heat inactivation is a simple step that 
      eliminates false-positive reactions in the B-cell flow cytometry crossmatch.
FAU - Ta, Mai
AU  - Ta M
AD  - Shands Hospital, and Department of Pathology, University of Florida College of 
      Medicine, Gainesville, Florida 32610, USA.
FAU - Scornik, Juan C
AU  - Scornik JC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (HLA-D Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - B-Lymphocytes/immunology
MH  - Flow Cytometry/*methods
MH  - HLA-D Antigens/*immunology
MH  - Histocompatibility Testing/methods
MH  - Hot Temperature
MH  - Humans
MH  - Immunoglobulin G/blood
MH  - Isoantibodies/analysis/*blood
MH  - Leukocytes/immunology
MH  - Sensitivity and Specificity
MH  - T-Lymphocytes/immunology
MH  - *Tissue Donors
EDAT- 2002/06/04 10:00
MHDA- 2002/06/27 10:01
CRDT- 2002/06/04 10:00
PHST- 2002/06/04 10:00 [pubmed]
PHST- 2002/06/27 10:01 [medline]
PHST- 2002/06/04 10:00 [entrez]
AID - 10.1097/00007890-200205270-00015 [doi]
PST - ppublish
SO  - Transplantation. 2002 May 27;73(10):1611-4. doi: 
      10.1097/00007890-200205270-00015.