PMID- 12042810
OWN - NLM
STAT- MEDLINE
DCOM- 20020626
LR  - 20220330
IS  - 1078-8956 (Print)
IS  - 1078-8956 (Linking)
VI  - 8
IP  - 6
DP  - 2002 Jun
TI  - Alternate replication in B cells and epithelial cells switches tropism of 
      Epstein-Barr virus.
PG  - 594-9
AB  - Epstein-Barr virus is ubiquitous and is causally implicated in lymphoid and 
      epithelial malignancies. Virus invades oropharyngeal mucosa and establishes 
      latency in B lymphocytes. Reactivating lymphocytes shed virus into saliva for 
      spread to new hosts. A complex of three virus glycoproteins, gH, gL and gp42, is 
      essential for entry. B-cell entry requires binding of gp42 to human leukocyte 
      antigen (HLA) class II whereas entry into epithelial cells lacking HLA class II 
      requires complexes without gp42. To accommodate infection of each, the virus 
      carries both three-part and two-part complexes. We show here that HLA class II in 
      the virus-producing cell alters the ratio of three-part to two-part complexes. As 
      a consequence, virus originating in epithelial cells efficiently infects B cells 
      whereas B-cell derived virus better infects epithelial cells. This molecular 
      switch is a novel strategy that could alter tropism of virus from epithelium to B 
      cells and then back to epithelium in a new host.
FAU - Borza, Corina M
AU  - Borza CM
AD  - School of Biological Sciences, University of Missouri-Kansas City, Kansas City, 
      Missouri, USA. borzac@umkc.edu
FAU - Hutt-Fletcher, Lindsey M
AU  - Hutt-Fletcher LM
LA  - eng
GR  - R01-AI20662/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - 0 (DNA, Viral)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - EC 2.7.1.21 (Thymidine Kinase)
SB  - IM
CIN - Nat Med. 2002 Jun;8(6):559-60. PMID: 12042800
MH  - Animals
MH  - B-Lymphocytes/immunology/*virology
MH  - Cell Line
MH  - DNA, Viral/analysis
MH  - Epithelial Cells/immunology/*virology
MH  - Gene Deletion
MH  - Herpesvirus 4, Human/genetics/*physiology
MH  - Histocompatibility Antigens Class II/analysis
MH  - Lymphocyte Activation
MH  - Thymidine Kinase/deficiency/genetics
MH  - Virus Replication/*physiology
MH  - Virus Shedding
EDAT- 2002/06/04 10:00
MHDA- 2002/06/27 10:01
CRDT- 2002/06/04 10:00
PHST- 2002/06/04 10:00 [pubmed]
PHST- 2002/06/27 10:01 [medline]
PHST- 2002/06/04 10:00 [entrez]
AID - nm0602-594 [pii]
AID - 10.1038/nm0602-594 [doi]
PST - ppublish
SO  - Nat Med. 2002 Jun;8(6):594-9. doi: 10.1038/nm0602-594.