PMID- 12044056
OWN - NLM
STAT- MEDLINE
DCOM- 20020613
LR  - 20191210
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 49
IP  - 1
DP  - 2002
TI  - Cytotoxic T lymphocyte precursor frequency analysis in the selection of HLA 
      matched unrelated donors for hematopoietic stem cell transplantation: the 
      correlation of CTLp frequency with HLA class I genotyping and aGVHD development.
PG  - 26-32
AB  - The selection of human leukocyte antigen (HLA) compatible unrelated donors for 
      hematopoietic stem cell transplantation (HSCT) is based on the direct genotyping 
      of HLA class I and class II alleles (HLA-A, -B, -C, -DRB1, -DQB1 loci). The 
      cellular test estimating the frequency of cytotoxic T lymphocyte precursors 
      (CTLp) has been included into the selection procedure of unrelated donors to 
      detect the class I alloreactivity and to predict acute graft versus host disease 
      (aGVHD) occurrence and severity. The relationship between HLA-A, -B, -C 
      high/medium resolution genotyping and CTLp activation was analysed in the cohort 
      of 78 unrelated donor/patient pairs indicated for HSCT. The high frequency of 
      CTLp (> 1:100,000) correlated significantly (p < or = 0.0002) with the 
      incompatibilities in alleles of HLA-A, -B, -C loci. Nevertheless, the results of 
      HLA-A, -B, -C genotyping and CTLp assay are not fully alternative, suggesting 
      that the CTLp test gives its specific information. The high CTLp frequency 
      (CTLpf) in 14/35 pairs fully matched by HLA class-I alleles genotyping could 
      reflect the influence of another factors upon the CTLp activation. On the 
      contrary, the low CTLp frequency values (< or = 1:100,000) found in 8/43 pairs 
      with existing HLA class-I alleles incompatibilities could indicate the 
      immunological permissivity of these particular mismatches. The clinical relevance 
      of the CTLp test for aGVHD prediction has been also analysed. The relationship 
      between CTLp activation in vitro and the incidence and severity of aGVHD was 
      evaluated in 37 patients who underwent allogeneic HSCT. The severe form of aGVHD 
      (grade III-IV) developed in 9 of 18 cases (50%) with the high pretransplant CTLpf 
      value. The patients with the low CTLpf (n = 19) suffered from the severe form of 
      aGVHD in 2 cases (10%) only, the remaining 17 patients from this group were 
      without aGVHD symptoms or developed only the mild form of aGVHD (I-II). The 
      relationship between CTLp results and the incidence and severity of aGVHD was 
      found statistically significant (p < or = 0.01).
FAU - Dolezalova, L
AU  - Dolezalova L
AD  - Institute of Hematology and Blood Transfusion, Prague, Czech Republic. 
      doli@uhkt.cz
FAU - Vrana, M
AU  - Vrana M
FAU - Dobrovolna, M
AU  - Dobrovolna M
FAU - Loudova, M
AU  - Loudova M
FAU - Cukrova, V
AU  - Cukrova V
FAU - Vitek, A
AU  - Vitek A
FAU - Sajdova, J
AU  - Sajdova J
FAU - Stary, J
AU  - Stary J
FAU - Sedlacek, P
AU  - Sedlacek P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
SB  - IM
MH  - Genes, MHC Class I/*genetics
MH  - Genotype
MH  - Graft vs Host Disease/*genetics/pathology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Leukocytes, Mononuclear
MH  - Predictive Value of Tests
MH  - *T-Lymphocyte Subsets
MH  - *T-Lymphocytes, Cytotoxic
MH  - Tissue Donors
EDAT- 2002/06/05 10:00
MHDA- 2002/06/14 10:01
CRDT- 2002/06/05 10:00
PHST- 2002/06/05 10:00 [pubmed]
PHST- 2002/06/14 10:01 [medline]
PHST- 2002/06/05 10:00 [entrez]
PST - ppublish
SO  - Neoplasma. 2002;49(1):26-32.