PMID- 12044522
OWN - NLM
STAT- MEDLINE
DCOM- 20021210
LR  - 20240109
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 36
IP  - 6
DP  - 2002 Jun
TI  - Phenotypic analysis of circulating and intrahepatic dendritic cell subsets in 
      patients with chronic liver diseases.
PG  - 734-41
AB  - BACKGROUND/AIMS: Dendritic cells (DCs) are the most potent professional 
      antigen-presenting cells. Although two subsets of circulating DCs, 
      lineage(-)CD11c(+)CD4(low) (CD11c(+)DCs) and lineage (-)CD11c(-)CD4(+)CD123(+) 
      (CD123(+)DCs) are identified in humans, the role of each DC subset in the 
      immunopathogenesis of liver diseases is unknown. METHODS: We examined the numbers 
      and activation status of each DC subset in the circulation and in the inflamed 
      livers in patients with chronic liver diseases by flow cytometry and 
      immunohistochemistry. RESULTS: The numbers of circulating CD11c(+)DCs were 
      inversely correlated with serum alanine aminotransferase (ALT) levels in patients 
      with chronic viral hepatitis, and that the expression of costimulatory molecules 
      on circulating CD11c(+)DCs in patients with chronic viral hepatitis was 
      significantly up-regulated in patients with high serum levels of ALT. Both DCs 
      are also identified in the livers by flow cytometry, and the expression of 
      costimulatory molecule CD40 on those DCs was significantly higher in liver DCs 
      than that in circulating DCs. Moreover, the ratios of CD11c(+)DCs/CD123(+)DCs 
      were higher in liver DCs (mean+/-SD, 7.2+/-6.0) than those of circulating DCs 
      (4.0+/-4.6). Immunohistochemically, CD11c(+) or CD123(+) cells and CD83(+) 
      activated DCs were observed mostly in portal areas with mononuclear cell 
      infiltration in various liver diseases. These overall data suggest that DCs, 
      especially CD11c(+)DCs, could be associated with the necroinflammatory response 
      in the liver of chronic viral liver diseases. CONCLUSIONS: DCs, especially 
      CD11c(+)DCs, may be involved in the immunopathogenesis of chronic liver diseases.
FAU - Kunitani, Hitoshi
AU  - Kunitani H
AD  - The Third Department of Internal Medicine, Toyama Medical and Pharmaceutical 
      University, 2630 Sugitani, Toyama 930-0194, Japan.
FAU - Shimizu, Yukihiro
AU  - Shimizu Y
FAU - Murata, Hiroyuki
AU  - Murata H
FAU - Higuchi, Kiyohiro
AU  - Higuchi K
FAU - Watanabe, Akiharu
AU  - Watanabe A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (IL3RA protein, human)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-3 Receptor alpha Subunit)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Interleukin-3)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alanine Transaminase/blood
MH  - Antigens, CD/analysis
MH  - B7-2 Antigen
MH  - CD11c Antigen/analysis
MH  - CD40 Antigens/analysis
MH  - Chronic Disease
MH  - Dendritic Cells/chemistry/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Immunoglobulins/analysis
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Interleukin-3 Receptor alpha Subunit
MH  - Liver/*chemistry/*cytology
MH  - Liver Diseases/*pathology
MH  - Male
MH  - Membrane Glycoproteins/analysis
MH  - Middle Aged
MH  - Receptors, Interleukin-3/analysis
MH  - CD83 Antigen
EDAT- 2002/06/05 10:00
MHDA- 2002/12/11 04:00
CRDT- 2002/06/05 10:00
PHST- 2002/06/05 10:00 [pubmed]
PHST- 2002/12/11 04:00 [medline]
PHST- 2002/06/05 10:00 [entrez]
AID - S0168827802000624 [pii]
AID - 10.1016/s0168-8278(02)00062-4 [doi]
PST - ppublish
SO  - J Hepatol. 2002 Jun;36(6):734-41. doi: 10.1016/s0168-8278(02)00062-4.