PMID- 12044528
OWN - NLM
STAT- MEDLINE
DCOM- 20021210
LR  - 20211203
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 36
IP  - 6
DP  - 2002 Jun
TI  - Gene amplification and mRNA and protein overexpression of c-erbB-2 (HER-2/neu) in 
      human intrahepatic cholangiocarcinoma as detected by fluorescence in situ 
      hybridization, in situ hybridization, and immunohistochemistry.
PG  - 780-5
AB  - BACKGROUND/AIMS: The human proto-oncogene c-erbB-2 (also called HER-2/neu) is 
      located on chromosome 17q21-22. There have been no studies on gene amplification 
      or mRNA expression of c-erbB-2 in human intrahepatic cholangiocarcinoma (CC) 
      hitherto. METHODS: We investigated c-erbB-2 gene amplification by fluorescence in 
      situ hybridization (FISH), c-erbB2 mRNA expression by ISH, and c-erbB-2 protein 
      expression by immunohistochemistry in 22 archival cases of CC. RESULTS: FISH 
      revealed that c-erbB-2 gene signals were increased in CC. ISH showed that 
      c-erbB-2 mRNA signals were located in the nuclei and cytoplasms of cancer cells 
      and were increased in cancer cells compared with non-cancerous bile ducts where 
      no signals were present. Immunohistochemistry showed that the c-erbB-2 protein 
      was expressed in the cell membrane of cancer cells, and was increased compared 
      with non-cancerous bile ducts where no expression was found. There was a positive 
      significant correlation between c-erbB-2 mRNA and protein expression. 
      Clinicopathologically, there were no correlations between the c-erbB-2 expression 
      and various pathological features. CONCLUSIONS: These data suggest that c-erbB-2 
      gene amplification does occur in CC, and that there is an overexpressed c-erbB-2 
      protein through the enhanced mRNA expression. The c-erbB-2 gene amplification may 
      be related to the oncogenesis or tumor progression of CC.
FAU - Ukita, Yoko
AU  - Ukita Y
AD  - Second Department of Pathology, Tottori University Faculty of Medicine, 86 
      Nishi-cho, Yonago 683-8503, Japan.
FAU - Kato, Masako
AU  - Kato M
FAU - Terada, Tadashi
AU  - Terada T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cholangiocarcinoma/chemistry/*physiopathology
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms/chemistry/*physiopathology
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Mas
MH  - RNA, Messenger/analysis
MH  - Receptor, ErbB-2/analysis/*genetics
EDAT- 2002/06/05 10:00
MHDA- 2002/12/11 04:00
CRDT- 2002/06/05 10:00
PHST- 2002/06/05 10:00 [pubmed]
PHST- 2002/12/11 04:00 [medline]
PHST- 2002/06/05 10:00 [entrez]
AID - S0168827802000570 [pii]
AID - 10.1016/s0168-8278(02)00057-0 [doi]
PST - ppublish
SO  - J Hepatol. 2002 Jun;36(6):780-5. doi: 10.1016/s0168-8278(02)00057-0.