PMID- 12048207
OWN - NLM
STAT- MEDLINE
DCOM- 20021024
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 38
DP  - 2002 Sep 20
TI  - Insulin and sterol-regulatory element-binding protein-1c (SREBP-1C) regulation of 
      gene expression in 3T3-L1 adipocytes. Identification of CCAAT/enhancer-binding 
      protein beta as an SREBP-1C target.
PG  - 35625-34
AB  - We evaluated the hypothesis of sterol-regulatory element-binding protein 
      (SREBP)-1c being a general mediator of the transcriptional effects of insulin, 
      with a focus on adipocytes, in which insulin profoundly influences specific gene 
      expression. Using real time quantitative reverse transcriptase-PCR to monitor 
      changes in the expression of about 50 genes that cover a wide range of adipocyte 
      functions, we have compared the impact of insulin treatment with that of 
      adenoviral overexpression of either dominant positive or dominant negative 
      SREBP-1c mutants in 3T3-L1 adipocytes. As expected, insulin up-regulated, 
      dominant positive stimulated, and dominant negative decreased previously 
      characterized direct SREBP targets (FAS, SCD-1, and low density lipoprotein 
      receptor). We also identified three novel SREBP-1c transcriptional targets in 
      adipocytes, which were confirmed by run-on assays: plasminogen activator 
      inhibitor 1, CCAAT/enhancer-binding protein delta (C/EBPdelta), and C/EBPbeta. 
      Because most insulin-regulated genes were also modulated by SREBP-1c mutants, our 
      data establish that 1) SREBP-1c is an important mediator of insulin 
      transcriptional effects in adipocytes, and 2) C/EBPbeta is under the direct 
      control of SREBP-1c, as demonstrated by the ability of SREBP-1c to activate the 
      transcription from C/EBPbeta promoter through canonical SREBP binding sites. 
      Thus, some of the effects of insulin and/or SREBP-1c in mature fat cells might 
      require C/EBPbeta or C/EBPdelta as transcriptional relays.
FAU - Le Lay, Soazig
AU  - Le Lay S
AD  - INSERM Unite 465, Centre de Recherches Biomedicales des Cordeliers, 15 rue de 
      l'Ecole de Medecine, 75270 Paris Cedex 06, France.
FAU - Lefrere, Isabelle
AU  - Lefrere I
FAU - Trautwein, Christian
AU  - Trautwein C
FAU - Dugail, Isabelle
AU  - Dugail I
FAU - Krief, Stephane
AU  - Krief S
LA  - eng
PT  - Journal Article
DEP - 20020604
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Insulin)
RN  - 0 (Srebf1 protein, mouse)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - CCAAT-Enhancer-Binding Protein-beta/*metabolism
MH  - CCAAT-Enhancer-Binding Proteins/metabolism/*physiology
MH  - DNA Primers
MH  - DNA-Binding Proteins/metabolism/*physiology
MH  - Gene Expression Regulation/*physiology
MH  - Insulin/*physiology
MH  - Mice
MH  - Sterol Regulatory Element Binding Protein 1
MH  - *Transcription Factors
MH  - Transcription, Genetic/physiology
EDAT- 2002/06/06 10:00
MHDA- 2002/10/31 04:00
CRDT- 2002/06/06 10:00
PHST- 2002/06/06 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/06/06 10:00 [entrez]
AID - S0021-9258(18)36803-0 [pii]
AID - 10.1074/jbc.M203913200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Sep 20;277(38):35625-34. doi: 10.1074/jbc.M203913200. Epub 2002 
      Jun 4.