PMID- 12048291
OWN - NLM
STAT- MEDLINE
DCOM- 20020808
LR  - 20220410
IS  - 1462-0324 (Print)
IS  - 1462-0324 (Linking)
VI  - 41
IP  - 6
DP  - 2002 Jun
TI  - Invasion of Salmonella into human intestinal epithelial cells is modulated by 
      HLA-B27.
PG  - 651-7
AB  - OBJECTIVE: To investigate the influence of the major histocompatibility complex 
      (MHC) class I molecule HLA-B27 on (i) the invasion of Salmonella and Yersinia 
      into human intestinal epithelial cells, (ii) the survival of intracellular 
      Salmonella in these cells, and (iii) the production of certain inflammatory 
      cytokines by the cells after Salmonella infection. METHODS: The human intestinal 
      epithelial cell line Henle-407 was transfected with HLA-B27 DNA. These cells and 
      HLA-B27-negative control cells were infected with Salmonella or Yersinia, and 
      viable intracellular bacteria were determined as colony-forming units. Cytokine 
      production was assayed with ELISA. RESULTS: Salmonella invaded HLA-B27-positive 
      Henle cells in higher numbers than HLA-B27-negative control cells. However, 
      HLA-B27 did not affect the invasion of Yersinia or the survival of the 
      intracellular bacteria in these intestinal epithelial cells. Salmonella infection 
      induced production of interleukin-8 (IL-8), IL-6 and monocyte chemotactic protein 
      1 (MCP-1) by Henle cells that was not affected by HLA-B27 in a specific way. 
      CONCLUSIONS: These findings suggest that HLA-B27 enhances the invasion of 
      Salmonella into intestinal epithelial cells. The interaction between bacteria and 
      intestinal epithelial cells plays an important role during the early phases of 
      ReA. HLA-B27-linked modulation of Salmonella invasion may lead to an increased 
      load of Salmonella in intestinal tissue and thus increased susceptibility to 
      reactive arthritis.
FAU - Saarinen, M
AU  - Saarinen M
AD  - National Public Health Institute, Department in Turku, Turku, Finland, UCLA 
      School of Medicine, Los Angeles, CA 90095, USA.
FAU - Ekman, P
AU  - Ekman P
FAU - Ikeda, M
AU  - Ikeda M
FAU - Virtala, M
AU  - Virtala M
FAU - Gronberg, A
AU  - Gronberg A
FAU - Yu, D T Y
AU  - Yu DT
FAU - Arvilommi, H
AU  - Arvilommi H
FAU - Granfors, K
AU  - Granfors K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (Cytokines)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Epithelial Cells/cytology/immunology/*microbiology
MH  - Gene Expression/immunology
MH  - HLA-B27 Antigen/genetics/*immunology
MH  - Humans
MH  - Intestinal Mucosa/*cytology/immunology
MH  - Prohibitins
MH  - Salmonella Infections/*immunology/microbiology/pathology
MH  - Salmonella enteritidis/immunology/*pathogenicity
MH  - Transfection
MH  - Virulence
EDAT- 2002/06/06 10:00
MHDA- 2002/08/09 10:01
CRDT- 2002/06/06 10:00
PHST- 2002/06/06 10:00 [pubmed]
PHST- 2002/08/09 10:01 [medline]
PHST- 2002/06/06 10:00 [entrez]
AID - 10.1093/rheumatology/41.6.651 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2002 Jun;41(6):651-7. doi: 10.1093/rheumatology/41.6.651.