PMID- 12051533
OWN - NLM
STAT- MEDLINE
DCOM- 20030108
LR  - 20191210
IS  - 0944-1174 (Print)
IS  - 0944-1174 (Linking)
VI  - 37
IP  - 5
DP  - 2002
TI  - Increased expression of tumor necrosis factor-alpha messenger RNA in the 
      intestinal mucosa of inflammatory bowel disease, particularly in patients with 
      disease in the inactive phase.
PG  - 345-53
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha), may be involved in the 
      pathogenesis of inflammatory bowel diseases (IBDs). The aim of this study was to 
      evaluate the effect of TNF-alpha on the inflammatory activity of IBD. METHODS: 
      TNF-alpha mRNA expression in intestinal mucosal biopsy specimens from IBD 
      patients [ulcerative colitis (UC), n = 54; and Crohn's disease (CD), n = 11] was 
      analyzed using a competitive polymerase chain reaction. The degree of macrophage 
      infiltration was analyzed by immunohistochemistry, using an antihuman CD68 
      antibody. RESULTS: TNF-alpha mRNA expression was increased in UC patients, 
      corresponding to the inflammatory activity. However, in CD, TNF-alpha mRNA 
      expression was not correlated with the endoscopic findings. CONCLUSIONS: We 
      clarified that TNF-alpha mRNA expression was responsible for the inflammatory 
      activity in UC. However, TNF-alpha mRNA expression was not correlated with the 
      mucosal injury in CD.
FAU - Akazawa, Akiko
AU  - Akazawa A
AD  - Department of Gastroenterology and Hepatology, Yamaguchi University, School of 
      Medicine, Ube, Japan.
FAU - Sakaida, Isao
AU  - Sakaida I
FAU - Higaki, Shingo
AU  - Higaki S
FAU - Kubo, Yoshitsugu
AU  - Kubo Y
FAU - Uchida, Koichi
AU  - Uchida K
FAU - Okita, Kiwamu
AU  - Okita K
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Japan
TA  - J Gastroenterol
JT  - Journal of gastroenterology
JID - 9430794
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, CD/*analysis
MH  - Antigens, Differentiation, Myelomonocytic/*analysis
MH  - Colitis, Ulcerative/*metabolism/pathology
MH  - Crohn Disease/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2002/06/08 10:00
MHDA- 2003/01/09 04:00
CRDT- 2002/06/08 10:00
PHST- 2002/06/08 10:00 [pubmed]
PHST- 2003/01/09 04:00 [medline]
PHST- 2002/06/08 10:00 [entrez]
AID - 10.1007/s005350200048 [doi]
PST - ppublish
SO  - J Gastroenterol. 2002;37(5):345-53. doi: 10.1007/s005350200048.