PMID- 12051640
OWN - NLM
STAT- MEDLINE
DCOM- 20021204
LR  - 20191106
IS  - 1541-2016 (Print)
IS  - 1533-4058 (Linking)
VI  - 10
IP  - 2
DP  - 2002 Jun
TI  - Detection of numerical chromosomal abnormalities in epithelial ovarian neoplasms 
      by fluorescence in situ hybridization (FISH) and a review of the current 
      literature.
PG  - 187-93
AB  - Preliminary retrospective chromosomal analysis was performed using fluorescence 
      in situ hybridization (FISH) with alphoid DNA probes for chromosomes 1, 3, 6, 8, 
      12, 17, and X. Twenty-four epithelial ovarian tumors were examined in this pilot 
      study, including 8 borderline (LMP) serous tumors, 9 serous carcinoma, and 7 
      mucinous carcinoma. Hybridization signals were counted to demonstrate the 
      frequency of aneusomy, trace chromosomal progression, and identify the 
      predominance of chromosome copy number abnormalities that are specific to a 
      particular histotype. The preliminary results revealed almost an equal number of 
      mean aneusomies in serous (58.13 +/- 13%) and mucinous (64.33 +/- 10%) carcinoma, 
      both of which were slightly higher than borderline serous tumors (50.57 +/- 17%). 
      Hyposomies 3 and X were significantly higher in mucinous than in serous ovarian 
      carcinomas, and lowest in borderline serous tumors (P<0.05 and P<0.01). Signal 
      losses were a more frequent abnormality in all three histologic subtypes. 
      Mucinous carcinomas showed a loss of chromosomes 8 (45.00 +/- 28%) and 3 (43.14 
      +/- 16%), in addition to a loss of chromosome X (56.29 +/- 12%). Serous 
      carcinomas showed a gain of chromosome 1 (39.44 +/- 32%), followed by losses of 
      chromosomes 6 (37.00 +/- 20%), 17 (36.44 +/- 19%), and 8 (36.89 +/- 19%). In 
      borderline serous tumors, the most frequent findings were losses of chromosomes 6 
      (38.00 +/- 17%), 12 (36.88 +/- 17%), and 3 (36.13 +/- 21%). However, further 
      research is necessary to substantiate these preliminary results and elucidate 
      their clinical significance. A brief review of the literature pertaining to 
      interphase cytogenetics in ovarian epithelial tumors is discussed also.
FAU - Huang, Ngan-Fong Tina
AU  - Huang NF
AD  - Maimonides Medical Center, Brooklyn, New York, USA.
FAU - Gupta, Mala
AU  - Gupta M
FAU - Varghese, Sara
AU  - Varghese S
FAU - Rao, Sujatha
AU  - Rao S
FAU - Luke, Sunny
AU  - Luke S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
SB  - IM
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Ovarian Neoplasms/*genetics/pathology
MH  - Pilot Projects
RF  - 37
EDAT- 2002/06/08 10:00
MHDA- 2002/12/05 04:00
CRDT- 2002/06/08 10:00
PHST- 2002/06/08 10:00 [pubmed]
PHST- 2002/12/05 04:00 [medline]
PHST- 2002/06/08 10:00 [entrez]
AID - 10.1097/00129039-200206000-00016 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2002 Jun;10(2):187-93. doi: 
      10.1097/00129039-200206000-00016.