PMID- 12052456 OWN - NLM STAT- MEDLINE DCOM- 20021210 LR - 20190906 IS - 8756-3282 (Print) IS - 1873-2763 (Linking) VI - 30 IP - 6 DP - 2002 Jun TI - Stage-dependent changes in trabecular bone turnover and osteogenic capacity of marrow cells during development of type II collagen-induced arthritis in mice. PG - 872-9 AB - Rheumatoid arthritis (RA) is a disease characterized by inflammatory polyarthritis leading to destruction of the joints and reduction in bone mass. However, the relationship between bone mass and turnover is not yet clear in RA patients. To clarify the effect of bone turnover and marrow osteogenic capacity on mass and structure during the development of arthritis, we examined DBA1/J mice for 8 weeks after the first immunization with bovine type II collagen at the age of 9 weeks. Localized arthritis developed at 4 weeks and advanced arthritis at 6 weeks postimmunization. Urinary deoxypyridinoline levels in arthritic mice were significantly higher at 4 weeks, and levels were maintained thereafter. Their serum osteocalcin levels were significantly reduced compared with controls at 2 and 6 weeks, but did not differ significantly from those in the control group at 4 and 8 weeks. Three-dimensional (3D) trabecular bone volume of the proximal tibia measured by 3D microcomputed tomography (micro-CT) in the arthritic mice became significantly lower at 4 weeks and decreased further at 6 weeks compared with controls. Parameters of 3D trabecular bone structure, such as structure model index and trabecular bone pattern factor, were increased at 4 and 6 weeks, respectively. Trabecular osteoclast number increased and bone formation rates decreased at 8 weeks. The number of total bone marrow cells (BMCs), adherent stromal cells, and area of mineralized nodule formation in the tibia of arthritic mice were significantly reduced compared with controls at 6 weeks. Numbers of total fibroblastic colony-forming units (CFU-f) and alkaline phosphatase (ALP)-positive CFU-f colonies also decreased. However, the values of these osteogenic parameters corrected for the total BMCs and/or adherent stromal cells did not differ significantly between the arthritic and control groups. These data indicate that an increase in bone resorption led to the reduction in trabecular bone mass and deterioration of 3D structure during the localized arthritic stage. The reduction in bone marrow osteogenic potential in the advanced arthritic stage was due to the reduction in the number of total bone marrow cells, and differentiation of osteogenic cells was apparently unaffected. The reduction in bone formation may not be substantial in this arthritic model. FAU - Nishida, S AU - Nishida S AD - Department of Orthopedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan. shige-n@med.uoeh-u.ac.jp FAU - Tsurukami, H AU - Tsurukami H FAU - Sakai, A AU - Sakai A FAU - Sakata, T AU - Sakata T FAU - Ikeda, S AU - Ikeda S FAU - Tanaka, M AU - Tanaka M FAU - Ito, M AU - Ito M FAU - Nakamura, T AU - Nakamura T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Bone JT - Bone JID - 8504048 RN - 0 (Autoantibodies) RN - 0 (Collagen Type II) SB - IM MH - Animals MH - Arthritis, Experimental/etiology/*metabolism/pathology/*physiopathology MH - Autoantibodies/biosynthesis MH - Body Weight MH - Bone Marrow Cells/*metabolism/pathology MH - Bone Remodeling/*physiology MH - Collagen Type II/*administration & dosage/immunology MH - Ear, External/pathology MH - Edema/etiology/pathology MH - Hypersensitivity, Delayed/etiology/pathology MH - Male MH - Mice MH - Mice, Inbred DBA MH - Osteogenesis/*physiology EDAT- 2002/06/08 10:00 MHDA- 2002/12/11 04:00 CRDT- 2002/06/08 10:00 PHST- 2002/06/08 10:00 [pubmed] PHST- 2002/12/11 04:00 [medline] PHST- 2002/06/08 10:00 [entrez] AID - S8756328202007251 [pii] AID - 10.1016/s8756-3282(02)00725-1 [doi] PST - ppublish SO - Bone. 2002 Jun;30(6):872-9. doi: 10.1016/s8756-3282(02)00725-1.