PMID- 12052566
OWN - NLM
STAT- MEDLINE
DCOM- 20020828
LR  - 20181130
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 33
IP  - 2
DP  - 2002 Jun 3
TI  - Identification of a chemotactic, MCP-1-like protein from Mycobacterium avium.
PG  - 115-24
AB  - In the immunocompetent host, Mycobacterium avium is responsible for chronic 
      localized pulmonary disease, which is characterized by the presence of increased 
      numbers of activated T cells and macrophages in the lungs. M. avium organisms as 
      well as sonic extracts of M. avium were found to act as chemoattractants for 
      THP-1 cells as well as monocytes, monocyte-derived macrophages and alveolar 
      macrophages obtained from normal human donors in an in vitro chemotaxis assay, 
      where a significantly higher number of cells were found in wells containing M. 
      avium compared to control wells. Proteolytic treatment of M. avium sonicate 
      resulted in significant loss (50%) of chemotactic activity. Monoclonal antibodies 
      against recombinant human monocyte chemoattractant protein-1 (MCP-1) were found 
      to cross-react with a 34-kDa protein of M. avium sonicate on Western blot and 
      inhibit M. avium sonicate-mediated chemotaxis of THP-1 cells (47%). These data 
      suggest the presence of an 'MCP-1 like' molecule on M. avium. Recruitment of host 
      immune regulatory cells to the site of infection by pathogens may be involved in 
      generating a local immune response or may be a bacterial strategy for survival 
      within the host by recruiting the cells that they infect, i.e. macrophages.
FAU - Rao, Savita P
AU  - Rao SP
AD  - Department of Medicine, Division of Pulmonary and Critical Care Medicine, 
      University of California San Diego, 200, W. Arbor Drive, San Diego, CA 
      92103-8374, USA. srao@ucsd.edu
FAU - Hayashi, Tomoko
AU  - Hayashi T
FAU - Catanzaro, Antonino
AU  - Catanzaro A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - *Chemotaxis, Leukocyte
MH  - Humans
MH  - Macrophages, Alveolar/*immunology/microbiology
MH  - Monocytes/*immunology/microbiology
MH  - Mycobacterium avium Complex/immunology/*metabolism
MH  - Mycobacterium avium-intracellulare Infection/microbiology
EDAT- 2002/06/08 10:00
MHDA- 2002/08/29 10:01
CRDT- 2002/06/08 10:00
PHST- 2002/06/08 10:00 [pubmed]
PHST- 2002/08/29 10:01 [medline]
PHST- 2002/06/08 10:00 [entrez]
AID - S0928824402002821 [pii]
AID - 10.1111/j.1574-695X.2002.tb00580.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2002 Jun 3;33(2):115-24. doi: 
      10.1111/j.1574-695X.2002.tb00580.x.