PMID- 12054588
OWN - NLM
STAT- MEDLINE
DCOM- 20020705
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 293
IP  - 1
DP  - 2002 Apr 26
TI  - Isolation of Chinese hamster ovary cell pex mutants: two PEX7-defective mutants.
PG  - 225-30
AB  - We searched for novel Chinese hamster ovary (CHO) cell mutants defective in 
      peroxisome biogenesis by an improved method using peroxisome targeting signal 2 
      (PTS2)-tagged enhanced green fluorescent protein (EGFP). From mutagenized TKaEG2 
      cells, the wild-type CHO-K1 stably expressing rat Pex2p and PTS2-EGFP, cell 
      colonies resistant to the 9-(1(')-pyrene)nonanol/ultraviolet treatment were 
      examined for intracellular location of PTS2-EGFP. Of six mutant cell clones two, 
      ZPEG227 and ZPEG231, showed cytosolic PTS2-EGFP, indicative of impaired PTS2 
      import, and numerous PTS1-positive particles. PEX7 expression restored the 
      impaired PTS2 import in both mutants. Cell fusion with fibroblasts from a patient 
      with PEX7-defective rhizomelic chondrodysplasia punctata did not complement PTS2 
      import defect of ZPEG227 and ZPEG231, confirming that these two are pex7 mutants. 
      Mutation analysis of PEX7 by reverse transriptase (RT)-PCR indicated that 
      ZPEG227-allele carried an inactivating nonsense mutation, Trp158Ter. Therefore, 
      ZPEG227 is a pex7 mutant possessing a newly identified mutation in mammalian pex7 
      cell lines.
FAU - Yanago, Eiko
AU  - Yanago E
AD  - Department of Biology, Faculty of Sciences, Kyushu University Graduate School, 
      Fukuoka 812-8581, Japan.
FAU - Hiromasa, Takahide
AU  - Hiromasa T
FAU - Matsumura, Tsuyoshi
AU  - Matsumura T
FAU - Kinoshita, Naohiko
AU  - Kinoshita N
FAU - Fujiki, Yukio
AU  - Fujiki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Luminescent Proteins)
RN  - 0 (PEX7 protein, human)
RN  - 0 (Peroxisomal Targeting Signal 2 Receptor)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.4.24.- (PHEX Phosphate Regulating Neutral Endopeptidase)
RN  - EC 3.4.24.- (PHEX protein, human)
RN  - EC 3.4.24.- (Phex protein, rat)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - CHO Cells
MH  - Cell Fusion
MH  - Cell Survival/radiation effects
MH  - Chondrodysplasia Punctata/genetics
MH  - Cricetinae
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/biosynthesis
MH  - Microbodies/genetics
MH  - Mutagenesis
MH  - *Mutation
MH  - Mutation, Missense
MH  - PHEX Phosphate Regulating Neutral Endopeptidase
MH  - Peroxisomal Targeting Signal 2 Receptor
MH  - Proteins/*genetics
MH  - Rats
MH  - Receptors, Cytoplasmic and Nuclear/*genetics
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Transfection
MH  - Ultraviolet Rays
MH  - Zellweger Syndrome/genetics
EDAT- 2002/06/11 10:00
MHDA- 2002/07/06 10:01
CRDT- 2002/06/11 10:00
PHST- 2002/06/11 10:00 [pubmed]
PHST- 2002/07/06 10:01 [medline]
PHST- 2002/06/11 10:00 [entrez]
AID - S0006-291X(02)00219-X [pii]
AID - 10.1016/S0006-291X(02)00219-X [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Apr 26;293(1):225-30. doi: 
      10.1016/S0006-291X(02)00219-X.