PMID- 12054635
OWN - NLM
STAT- MEDLINE
DCOM- 20020705
LR  - 20041117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 293
IP  - 1
DP  - 2002 Apr 26
TI  - Affinity improvement of the high-affinity immunoglobulin E receptor by phage 
      display.
PG  - 542-8
AB  - The immunoglobulin E (IgE)-binding site of its high-affinity receptor is 
      localized in the second immunoglobulin-like domain (D2) of the alpha-subunit (Fc 
      epsilon RI alpha). In this study, the randomized pentapeptides were introduced 
      between Glu(132) and Ile(138) of Fc epsilon RI alpha D2 and displayed on a 
      filamentous phage. After eight rounds of panning, a phage clone having a mutation 
      of Asp(135)Tyr(136)Met(137) in Fc epsilon RI alpha D2 was obtained. The binding 
      affinity of the mutant phages to immobilized IgE was approximately 500 times 
      higher than that of the wild type. The mutant phages competitively inhibited the 
      binding of IgE to the soluble receptor at a 50% inhibition (IC(50)) value of 116 
      pM. The mutant Fc epsilon RI alpha D2, which had been expressed as a fusion 
      protein with glutathione S-transferase in Escherichia coli, also showed higher 
      IgE-binding capacity than the wild type. The mutant Fc epsilon RI alpha D2 is 
      expected to manifest its improved IgE-binding affinity together with any fusion 
      partner.
FAU - Iwasaki, Akio
AU  - Iwasaki A
AD  - Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, 
      Tsukuba, Ibaraki 305-8572, Japan.
FAU - Doi, Takeshi
AU  - Doi T
FAU - Umetani, Michihisa
AU  - Umetani M
FAU - Watanabe, Masanao
AU  - Watanabe M
FAU - Suda, Makoto
AU  - Suda M
FAU - Hattori, Yukio
AU  - Hattori Y
FAU - Nagoya, Takao
AU  - Nagoya T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Peptide Library)
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Base Sequence
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - *Peptide Library
MH  - Polymerase Chain Reaction
MH  - Receptors, IgE/antagonists & inhibitors/genetics/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
EDAT- 2002/06/11 10:00
MHDA- 2002/07/06 10:01
CRDT- 2002/06/11 10:00
PHST- 2002/06/11 10:00 [pubmed]
PHST- 2002/07/06 10:01 [medline]
PHST- 2002/06/11 10:00 [entrez]
AID - S0006-291X(02)00261-9 [pii]
AID - 10.1016/S0006-291X(02)00261-9 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Apr 26;293(1):542-8. doi: 
      10.1016/S0006-291X(02)00261-9.