PMID- 12058450
OWN - NLM
STAT- MEDLINE
DCOM- 20021127
LR  - 20190910
IS  - 0388-1350 (Print)
IS  - 0388-1350 (Linking)
VI  - 27
IP  - 2
DP  - 2002 May
TI  - Effects on fetal thymocyte populations and postnatal T-cell-dependent immune 
      functions after maternal exposure to 5-fluorouracil during pregnancy in mice.
PG  - 79-86
AB  - 5-Fluorouracil (5-FU) is a cytostatic anti-tumor drug which is known to have 
      immunosuppressive activities. To assess the immunotoxic effects of 5-FU on fetal 
      thymocyte populations and immune functions after birth, pregnant C57BL/6 mice 
      were orally administered vehicle or 17 mg/kg/day of 5-FU during gestational days 
      (GD) from 6 to 14. The fetal thymocyte populations were analyzed with flow 
      cytometry (CD4/CD8 double staining), and immune functions (a mixed lymphocyte 
      reaction, in vitro cytotoxic T-cell response, in vitro antibody-forming response) 
      after birth were measured. Fetal thymus weight and thymocyte numbers were 
      decreased by 5-FU administration. The decrease of the thymocytes was due mainly 
      to the decrease of small CD4CD8 double positive (DP) thymocytes. The thymocyte 
      numbers and populations recovered to the normal level 1 week after birth. The 
      mixed lymphocyte response at the 6th week after birth tended to be slightly lower 
      than the control levels, but the cytotoxic T-cell response and the 
      antibody-forming response were the same as the control levels. These results 
      suggest that immune functions might recover after birth, although maternal 
      administration of 5-FU has a suppressive effect on fetal thymocyte maturation.
FAU - Inoue, Tomoaki
AU  - Inoue T
AD  - Department of Preclinical Science, Nippon Roche Research Center, 200 Kajiwara, 
      Kamakura, Kanagawa 247-8530, Japan.
FAU - Horii, Ikuo
AU  - Horii I
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Toxicol Sci
JT  - The Journal of toxicological sciences
JID - 7805798
RN  - 0 (Immunosuppressive Agents)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Body Weight/drug effects
MH  - Female
MH  - Flow Cytometry
MH  - Fluorouracil/administration & dosage/*toxicity
MH  - Immune System/*drug effects/immunology
MH  - Immunosuppressive Agents/administration & dosage/*toxicity
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organ Size/drug effects
MH  - Organogenesis/*drug effects/immunology
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects
MH  - T-Lymphocytes/*drug effects/immunology
MH  - Thymus Gland/*drug effects/embryology/immunology/pathology
EDAT- 2002/06/13 10:00
MHDA- 2002/11/28 04:00
CRDT- 2002/06/13 10:00
PHST- 2002/06/13 10:00 [pubmed]
PHST- 2002/11/28 04:00 [medline]
PHST- 2002/06/13 10:00 [entrez]
AID - 10.2131/jts.27.79 [doi]
PST - ppublish
SO  - J Toxicol Sci. 2002 May;27(2):79-86. doi: 10.2131/jts.27.79.