PMID- 12060120
OWN - NLM
STAT- MEDLINE
DCOM- 20020910
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 117
IP  - 4
DP  - 2002 Jun
TI  - Chromosomal aberrations are shared by malignant plasma cells and a small fraction 
      of circulating CD19+ cells in patients with myeloma and monoclonal gammopathy of 
      undetermined significance.
PG  - 852-9
AB  - In the present study, we aimed to identify distinct structural and numerical 
      chromosomal aberrations in peripheral blood B cells of patients with myeloma and 
      monoclonal gammopathy of undetermined significance (MGUS), which reflect changes 
      thought to occur at different stages of the disease process. Peripheral blood 
      from 12 patients with multiple myeloma and three patients with MGUS was 
      investigated for the occurrence of retinoblastoma-1 gene deletions, p53 gene 
      deletions and numerical aberrations demonstrated previously to be present in the 
      patients' bone marrow CD138+ cells. By combining immunocytochemical staining for 
      light chains and interphase fluorescence in situ hybridization (FISH), aberrant 
      light-chain +ve cells were detected in the circulating CD19+ cell fraction. Each 
      kind of chromosomal change present in the myeloma tumour cells was found to be 
      shared by a small fraction of CD19+ cells (0.1-1.8%; median 0.36%, n = 6). In one 
      MGUS patient, aberrant cells could be identified with a frequency of 0.34% within 
      the CD19-sorted cell fraction. Clonotypic cells were detected with a frequency of 
      0.01-0.07% of peripheral blood nucleated cells by m-RNA in situ hybridization 
      with patient-specific probes in three investigated patients. These results 
      provide evidence that the circulating clonotypic B cells are closely related to 
      the malignant plasma cells in myeloma and MGUS.
FAU - Zojer, Niklas
AU  - Zojer N
AD  - First Department of Internal Medicine and Medical Oncology, Wilhelminenspital, 
      University of Vienna, Montleartstrasse 37, 1160 Vienna, Austria.
FAU - Schuster-Kolbe, Judith
AU  - Schuster-Kolbe J
FAU - Assmann, Irene
AU  - Assmann I
FAU - Ackermann, Jutta
AU  - Ackermann J
FAU - Strasser, Kathrin
AU  - Strasser K
FAU - Hubl, Wolfgang
AU  - Hubl W
FAU - Drach, Johannes
AU  - Drach J
FAU - Ludwig, Heinz
AU  - Ludwig H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Antigens, CD19)
RN  - 0 (Disulfides)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 0 (SDC1 protein, human)
RN  - 0 (Syndecan-1)
RN  - 0 (Syndecans)
RN  - 135949-60-9 (RB 101)
RN  - 47E5O17Y3R (Phenylalanine)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD19/*immunology
MH  - Bone Marrow Examination
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 17
MH  - Clone Cells
MH  - Disease Progression
MH  - Disulfides
MH  - Gene Deletion
MH  - Genes, Immunoglobulin
MH  - Genes, Retinoblastoma
MH  - Genes, p53
MH  - Humans
MH  - Immunoglobulin G
MH  - Immunoglobulin Light Chains
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Membrane Glycoproteins
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics/immunology
MH  - Paraproteinemias/*genetics/immunology
MH  - Phenylalanine/*analogs & derivatives
MH  - Plasma Cells/*physiology
MH  - Polymerase Chain Reaction/methods
MH  - Proteoglycans
MH  - RNA, Messenger/analysis
MH  - Syndecan-1
MH  - Syndecans
MH  - T-Lymphocytes/immunology/*physiology
EDAT- 2002/06/13 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/06/13 10:00
PHST- 2002/06/13 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/06/13 10:00 [entrez]
AID - 3529 [pii]
AID - 10.1046/j.1365-2141.2002.03529.x [doi]
PST - ppublish
SO  - Br J Haematol. 2002 Jun;117(4):852-9. doi: 10.1046/j.1365-2141.2002.03529.x.