PMID- 12060529
OWN - NLM
STAT- MEDLINE
DCOM- 20030916
LR  - 20061115
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 23
IP  - 6
DP  - 2002 Jun
TI  - Effects of Coriolus versicolor polysaccharide B on monocyte chemoattractant 
      protein 1 gene expression in rat.
PG  - 539-43
AB  - AIM: To investigate the effect of Coriolus versicolor polysaccharide B (CVPS-B), 
      a new water-soluble component of polysaccharides from the fungus Coriolus 
      versicolor (Fr) L on monocyte chemoattractant protein-1 (MCP-1) gene expression 
      in rat splenocytes. METHODS: Expression of MCP-1 mRNA in rat splenocytes was 
      examined by reverse transcription-polymerase chain reaction (RT-PCR) with beta- 
      actin as an internal standard. Sequencing of RT-PCR products was performed to 
      confirm their specificity in MCP-1 gene composition. RESULTS: (1) Without 
      pre-treatment of lipopolysaccharide (LPS), the relative MCP-1 mRNA expression 
      ratios (MCP-1/beta-actin) for the saline control group and for CVPS-B groups in 3 
      different doses (10, 20, and 30 mg . kg-1 . d-1, ip, for 4 d) were 1.4 +/- 0.3, 
      1.6 +/- 0.4, 1.7 +/- 0.5, and 1.5 +/- 0.4, respectively (P > 0.05); (2) LPS (10 
      microg . kg-1, ip) enhanced the expression of MPC-1 mRNA by the ratio of 114 %; 
      (3) pre-treatment with CVPS-B of 4 different doses (5, 10, 30, and 50 mg . kg-1 . 
      d-1, ip, for 4 d) decreased the LPS induced expression of MPC-1 mRNA by the 
      ratios of 51 %, 70 %, 84 %, and 99 %, respectively (n = 6). CONCLUSION: In a 
      dose-related fashion, CVPS-B inhibited the expression of MCP-1 mRNA induced by 
      LPS in the rat splenocytes, but did not significantly affect the expression of 
      MPC-1 mRNA in the normal rat.
FAU - Song, Lie-Chang
AU  - Song LC
AD  - Laboratory for Molecular Pharmacology, Department of Hygiology, First Military 
      Medical University, Guangzhou 510515, China. songlc@fimmu.edu.cn
FAU - Chen, Hai-Sheng
AU  - Chen HS
FAU - Lou, Ning
AU  - Lou N
FAU - Song, Chang
AU  - Song C
FAU - Zeng, Jun
AU  - Zeng J
FAU - Fu, Ting-Huan
AU  - Fu TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Dose-Response Relationship, Drug
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Polyporales/*chemistry
MH  - Polysaccharides/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Spleen/cytology/metabolism
EDAT- 2002/06/13 10:00
MHDA- 2003/09/17 05:00
CRDT- 2002/06/13 10:00
PHST- 2002/06/13 10:00 [pubmed]
PHST- 2003/09/17 05:00 [medline]
PHST- 2002/06/13 10:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2002 Jun;23(6):539-43.