PMID- 12061639
OWN - NLM
STAT- MEDLINE
DCOM- 20030128
LR  - 20131121
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 46
IP  - 2
DP  - 2002 Apr-Jun
TI  - Latency sites and reactivation of duck enteritis virus.
PG  - 308-13
AB  - Duck virus enteritis (DVE) is a contagious disease caused by herpesvirus in 
      waterfowl populations. Recovered birds become carriers and shed the virus 
      periodically. Reactivation of latent duck enteritis virus (DEV) has been 
      implicated in outbreaks of DVE in domestic and migrating waterfowl populations. 
      In this study, the sites for virus latency were determined in white Pekin ducks 
      infected with the DEV-97 strain. At 3 wk postinfection, infectious virus was not 
      detectable in tissues or cloacal swabs (CSs). At 7 and 9 weeks postinfection, the 
      viral DNA was detected by polymerase chain reaction in the trigeminal ganglia 
      (TG), suggesting that the virus is latent. Viral DNA was detected in the 
      peripheral blood lymphocytes (PBL), spleen, thymus, bursa, and CSs only after in 
      vitro cocultivation. In vivo virus reactivation was demonstrated when 
      dexamethasone or a combination of dexamethasone and cyclophosphamide was 
      inoculated in latently infected ducks. The reactivation of DEV occurred without 
      any clinical evidence of the disease, but the virus was detected in PBL and CSs. 
      We conclude from this study that DEV establishes latency in TG and lymphoid 
      tissues including PBL.
FAU - Shawky, Samia
AU  - Shawky S
AD  - Cornell Duck Research Laboratory, Eastport, NY 11941, USA.
FAU - Schat, Karel A
AU  - Schat KA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (DNA, Viral)
RN  - 0 (Glucocorticoids)
RN  - 0 (Immunosuppressive Agents)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Carrier State/veterinary/virology
MH  - Cells, Cultured
MH  - Cloaca/virology
MH  - Cyclophosphamide/pharmacology
MH  - DNA, Viral/isolation & purification
MH  - Dexamethasone/pharmacology
MH  - *Ducks/embryology
MH  - Glucocorticoids/pharmacology
MH  - Herpesviridae/genetics/pathogenicity/*physiology
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Immunosuppressive Agents/pharmacology
MH  - Liver/cytology/embryology/virology
MH  - Lymphoid Tissue/virology
MH  - Poultry Diseases/*virology
MH  - Specific Pathogen-Free Organisms
MH  - Trigeminal Ganglion/*virology
MH  - Virulence
MH  - Virus Activation/drug effects
MH  - Virus Latency
MH  - Virus Shedding
EDAT- 2002/06/14 10:00
MHDA- 2003/01/29 04:00
CRDT- 2002/06/14 10:00
PHST- 2002/06/14 10:00 [pubmed]
PHST- 2003/01/29 04:00 [medline]
PHST- 2002/06/14 10:00 [entrez]
AID - 10.1637/0005-2086(2002)046[0308:LSAROD]2.0.CO;2 [doi]
PST - ppublish
SO  - Avian Dis. 2002 Apr-Jun;46(2):308-13. doi: 
      10.1637/0005-2086(2002)046[0308:LSAROD]2.0.CO;2.