PMID- 12062469
OWN - NLM
STAT- MEDLINE
DCOM- 20020807
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 935
IP  - 1-2
DP  - 2002 May 10
TI  - Alterations in hippocampal GAP-43, BDNF, and L1 following sustained cerebral 
      ischemia.
PG  - 24-31
AB  - Alterations in factors involved in the regeneration of the neuronal network in 
      the hippocampus of rats with microsphere embolism (ME) were examined. Nine 
      hundred microspheres (48 microm in diameter) were injected into the right 
      hemisphere, and immunochemical and immunohistochemical studies on the hippocampus 
      were performed on the seventh day thereafter. Hematoxylin-eosin staining showed 
      progressive and severe degeneration of the hippocampus after ME. The protein 
      levels of brain-derived neurotrophic factor (BDNF), 43-kDa growth-associated 
      protein (GAP-43), and adhesion protein L1 (L1) in the ipsilateral hippocampus of 
      the ME animal, determined by Western blot analysis or enzyme immunoassay, were 
      increased, unaltered, and decreased, respectively. In contrast, the 
      immunohistochemical study showed increases in a marker of axonal sprouting 
      GAP-43, and a neurotrophic factor BDNF, and a decrease in an adhesion molecule L1 
      in some areas of the hippocampal ischemic penumbra of such animals. These results 
      suggest that some factors for regeneration of the neuronal network in the 
      ischemic penumbra responded to sustained cerebral ischemia for a certain period, 
      although functional network of the nerve cells in the microsphere-injected 
      hemisphere would be unlikely established after ME.
FAU - Miyake, Keiko
AU  - Miyake K
AD  - Department of Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 
      Horinouchi, 192-0392 Hachioji, Japan.
FAU - Yamamoto, Wataru
AU  - Yamamoto W
FAU - Tadokoro, Mina
AU  - Tadokoro M
FAU - Takagi, Norio
AU  - Takagi N
FAU - Sasakawa, Kyoko
AU  - Sasakawa K
FAU - Nitta, Atsumi
AU  - Nitta A
FAU - Furukawa, Shoei
AU  - Furukawa S
FAU - Takeo, Satoshi
AU  - Takeo S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Leukocyte L1 Antigen Complex)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neural Cell Adhesion Molecules)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*metabolism/pathology/physiopathology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Carotid Artery Thrombosis/metabolism/pathology/physiopathology
MH  - Cell Death/physiology
MH  - Cerebral Infarction/*metabolism/pathology/physiopathology
MH  - Cerebrovascular Circulation/physiology
MH  - Disease Models, Animal
MH  - Down-Regulation/physiology
MH  - GAP-43 Protein/*metabolism
MH  - Hippocampus/*metabolism/pathology/physiopathology
MH  - Immunohistochemistry
MH  - Leukocyte L1 Antigen Complex
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Microspheres
MH  - Nerve Degeneration/metabolism/pathology/physiopathology
MH  - Nerve Regeneration/*physiology
MH  - Neural Cell Adhesion Molecules/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Up-Regulation/physiology
EDAT- 2002/06/14 10:00
MHDA- 2002/08/08 10:01
CRDT- 2002/06/14 10:00
PHST- 2002/06/14 10:00 [pubmed]
PHST- 2002/08/08 10:01 [medline]
PHST- 2002/06/14 10:00 [entrez]
AID - S0006899302024204 [pii]
AID - 10.1016/s0006-8993(02)02420-4 [doi]
PST - ppublish
SO  - Brain Res. 2002 May 10;935(1-2):24-31. doi: 10.1016/s0006-8993(02)02420-4.