PMID- 12063401
OWN - NLM
STAT- MEDLINE
DCOM- 20020711
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 95
IP  - 3-4
DP  - 2001
TI  - Analysis of chromosomal aberrations involving chromosome 1q31-->q53 in a 
      DMBA-induced rat fibrosarcoma cell line: amplification and overexpression of 
      Jak2.
PG  - 202-9
AB  - In a study of DMBA-induced rat fibrosarcomas we repeatedly found deletions and/or 
      amplifications in the long arm of rat chromosome 1 (RNO1). Comparative genome 
      hybridization showed that there was amplification involving RNO1q31-->q53 in one 
      of the DMBA-induced rat fibrosarcoma tumors (LB31) and a cell culture derived 
      from it. To identify the amplified genes we physically mapped rat genes 
      implicated in cancer and analyzed them for signs of amplification. The genes were 
      selected based on their locations in comparative maps between rat and man. The 
      rat proto-oncogenes Ccnd1, Fgf4, and Fgf3 (HSA11q13.3), were mapped to RNO1q43 by 
      fluorescence in situ hybridization (FISH). The Ems1 gene was mapped by radiation 
      hybrid (RH) mapping to the same rat chromosome region and shown to be situated 
      centromeric to Ccnd1 and Fgf4. In addition, the proto-oncogenes Hras (HSA11p15.5) 
      and Igf1r (HSA15q25-->q26) were mapped to RNO1q43 and RNO1q32 by FISH and Omp 
      (HSA11q13.5) was assigned to RNO1q34. PCR probes for the above genes together 
      with PCR probes for the previously mapped rat genes Bax (RNO1q31) and Jak2 
      (RNO1q51-->q53) were analyzed for signs of amplification by Southern blot 
      hybridization. Low copy number increases of the Omp and Jak2 genes were detected 
      in the LB31 cell culture. Dual color FISH analysis of tumor cells confirmed that 
      chromosome regions containing Omp and Jak2 were amplified and were situated in 
      long marker chromosomes showing an aberrant banding pattern. The configuration of 
      the signals in the marker chromosomes suggested that they had arisen by a 
      break-fusion-bridge (BFB) mechanism.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Sjoling, A
AU  - Sjoling A
AD  - Department of Cell and Molecular Biology-Genetics, Goteborg University, Goteborg, 
      Sweden. Asa.Sjoling@gen.gu.se
FAU - Lindholm, H
AU  - Lindholm H
FAU - Samuelson, E
AU  - Samuelson E
FAU - Yamasaki, Y
AU  - Yamasaki Y
FAU - Watanabe, T K
AU  - Watanabe TK
FAU - Tanigami, A
AU  - Tanigami A
FAU - Levan, G
AU  - Levan G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Carcinogens)
RN  - 0 (Cortactin)
RN  - 0 (Cttn protein, mouse)
RN  - 0 (Cttn protein, rat)
RN  - 0 (Fgf3 protein, mouse)
RN  - 0 (Fgf3 protein, rat)
RN  - 0 (Fibroblast Growth Factor 3)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (fibroblast growth factor 14)
RN  - 136601-57-5 (Cyclin D1)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Jak2 protein, mouse)
RN  - EC 2.7.10.2 (Jak2 protein, rat)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene
MH  - Animals
MH  - Carcinogens
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Cortactin
MH  - Cyclin D1/genetics
MH  - Fibroblast Growth Factor 3
MH  - Fibroblast Growth Factors/genetics
MH  - Fibrosarcoma/chemically induced/*genetics
MH  - Gene Amplification
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - In Situ Hybridization, Fluorescence
MH  - Janus Kinase 2
MH  - Mice
MH  - Microfilament Proteins/genetics
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Proto-Oncogene Proteins/genetics
MH  - Rats
MH  - Rats, Inbred BN
MH  - Tumor Cells, Cultured
EDAT- 2002/06/14 10:00
MHDA- 2002/07/12 10:01
CRDT- 2002/06/14 10:00
PHST- 2002/06/14 10:00 [pubmed]
PHST- 2002/07/12 10:01 [medline]
PHST- 2002/06/14 10:00 [entrez]
AID - 59347 [pii]
AID - 10.1159/000059347 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2001;95(3-4):202-9. doi: 10.1159/000059347.