PMID- 12064459
OWN - NLM
STAT- MEDLINE
DCOM- 20020705
LR  - 20131121
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 191
IP  - 2
DP  - 2002 May
TI  - Stimulation of the proliferation and differentiation of mouse pink-eyed dilution 
      epidermal melanocytes by excess tyrosine in serum-free primary culture.
PG  - 162-72
AB  - The epidermal cell suspensions of the neonatal dorsal skin derived from wild type 
      mouse at the pink-eyed dilution (p) locus (black, C57BL/10JHir-P/P) and their 
      congenic mutant mouse (pink-eyed dilution, C57BL/10JHir-p/p) were cultured with a 
      serum-free melanocyte growth medium supplemented with additional L-tyrosine (Tyr) 
      from initiation of the primary culture. L-Tyr inhibited the proliferation of 
      P/Pmelanocytes in a dose-dependent manner, whereas L-Tyr stimulated the 
      proliferation of p/p melanoblasts and melanocytes regardless of dose. On the 
      other hand, L-Tyr stimulated (P/P) or induced (p/p) the differentiation of 
      epidermal melanocytes in a dose-dependent manner. In both P/P and p/p 
      melanoblasts and melanocytes cultured with 2.0 mM L-Tyr for 14 days, slight 
      increases in contents of eumelanin marker, pyrrole-2,3,5-tricarboxylic acid 
      (PTCA) and pheomelanin marker, aminohydroxyphenylalanine (AHP) were observed. The 
      average number of total melanosomes (stages I, II, III, and IV) per P/P 
      melanocyte was not changed by L-Tyr treatment, but the proportion of stage IV 
      melanosomes in the total melanosomes was increased. On the contrary, in p/p 
      melanoblasts and melanocytes L-Tyr increased dramatically the number of stage II, 
      III, and IV melanosomes as well as the proportion of stage III melanosomes. 
      Contents of PTCA and eumelanin precursor, 5,6-dihydroxyindole-2-carboxylic acid 
      (DHICA) of cultured media in p/p melanocytes were much more greatly increased 
      than in P/P melanocytes. However, contents of AHP and pheomelanin precursor, 
      5-S-cysteinyldopa (5-S-CD) of cultured media in p/p melanocytes were increased in 
      a similar tendency to P/Pmelanocytes. These results suggest that p/p melanocytes 
      in the primary culture are induced to synthesize eumelanin by excess L-Tyr, but 
      difficult to accumulate them in melanosomes.
FAU - Hirobe, Tomohisa
AU  - Hirobe T
AD  - Radiation Hazards Research Group, National Institute of Radiological Sciences, 
      Anagawa, Chiba, Japan. thirobe@nirs.go.jp
FAU - Wakamatsu, Kazumasa
AU  - Wakamatsu K
FAU - Ito, Shosuke
AU  - Ito S
FAU - Abe, Hiroyuki
AU  - Abe H
FAU - Kawa, Yoko
AU  - Kawa Y
FAU - Mizoguchi, Masako
AU  - Mizoguchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (5-S-cysteinyl-3,4-dihydroxyphenylalanine)
RN  - 0 (Carrier Proteins)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (Pyrroles)
RN  - 0 (pheomelanin)
RN  - 148710-77-4 (P protein, mouse)
RN  - 42HK56048U (Tyrosine)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
RN  - 63-84-3 (Dihydroxyphenylalanine)
RN  - 945-32-4 (pyrrole-2,3,5-tricarboxylic acid)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (Tyrp1 protein, mouse)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - *Carrier Proteins
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cell Division/drug effects/*physiology
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free/pharmacology
MH  - Dihydroxyphenylalanine/*analogs & derivatives/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Epidermis/drug effects/*metabolism/ultrastructure
MH  - Female
MH  - Indoles/metabolism
MH  - Male
MH  - Melanins/metabolism
MH  - Melanocytes/drug effects/*metabolism/ultrastructure
MH  - *Membrane Glycoproteins
MH  - Membrane Proteins/drug effects/*genetics/metabolism
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Monophenol Monooxygenase/metabolism
MH  - *Oxidoreductases
MH  - Proteins/metabolism
MH  - Proto-Oncogene Proteins c-kit/metabolism
MH  - Pyrroles/metabolism
MH  - Tyrosine/drug effects/*metabolism
EDAT- 2002/06/18 10:00
MHDA- 2002/07/06 10:01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/07/06 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
AID - 10.1002/jcp.10085 [doi]
PST - ppublish
SO  - J Cell Physiol. 2002 May;191(2):162-72. doi: 10.1002/jcp.10085.