PMID- 12064807
OWN - NLM
STAT- MEDLINE
DCOM- 20020625
LR  - 20190721
IS  - 0163-2116 (Print)
IS  - 0163-2116 (Linking)
VI  - 47
IP  - 6
DP  - 2002 Jun
TI  - Tumor necrosis factor-alpha mediates inhibitory effect of lipopolysaccharide on 
      L-leucine intestinal uptake.
PG  - 1316-22
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been proposed as an early proximal 
      mediator of many metabolic and physiologic responses during septic shock. We have 
      previously shown that direct addition to tissue (local effect) or intravenous 
      administration (systemic effect) of lipopolysaccharide (LPS) reduces L-leucine 
      absorption across rabbit jejunum. In the present study, we investigated whether 
      the inhibitory effect of LPS on L-leucine intestinal absorption in rabbit is 
      related to TNF-alpha. The results shown that the addition of TNF-alpha to tissue 
      does not produce any effect on L-leucine uptake by the enterocyte. When TNF-alpha 
      was inoculated by intravenous administration, a strong inhibition on the 
      L-leucine uptake (about 40%), mediated by a secretagogue effect on water and 
      Cl-ions was induced. We also found that the LPS intestinal effect induced by 
      intravenous administration, was blocked by a TNF-alpha antagonist, indicating 
      that TNF-alpha is a mediator of the LPS systemic effect on L-leucine intestinal 
      uptake inhibition. The study of possible mediators involved in the TNF-alpha 
      effect showed that nitric oxide and prostaglandins are implicated in the 
      L-leucine intestinal uptake.
FAU - Abad, B
AU  - Abad B
AD  - Veterinry Faculty, Department of Pharmacology and Physiology, University of 
      Zaragoza, Spain.
FAU - Mesonero, J E
AU  - Mesonero JE
FAU - Salvador, M T
AU  - Salvador MT
FAU - Garcia-Herrera, J
AU  - Garcia-Herrera J
FAU - Rodriguez-Yoldi, M J
AU  - Rodriguez-Yoldi MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Animals
MH  - Enterocytes/metabolism
MH  - Intestinal Absorption/drug effects/*physiology
MH  - Leucine/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Rabbits
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2002/06/18 10:00
MHDA- 2002/06/26 10:01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/06/26 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
AID - 10.1023/a:1015374631385 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2002 Jun;47(6):1316-22. doi: 10.1023/a:1015374631385.