PMID- 12065531
OWN - NLM
STAT- MEDLINE
DCOM- 20020730
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 70
IP  - 7
DP  - 2002 Jul
TI  - Hierarchy of susceptibility of dendritic cell subsets to infection by Leishmania 
      major: inverse relationship to interleukin-12 production.
PG  - 3874-80
AB  - Dendritic cells (DCs) are professional antigen-presenting cells which initiate 
      and regulate T-cell immune responses. Here we show that murine splenic DCs can be 
      ranked on the basis of their ability to phagocytose and harbor the obligately 
      intracellular parasite Leishmania major. CD4(+) CD8(-) DCs are the most 
      permissive host cells for L. major amastigotes, followed by CD4(-) CD8(-) DCs; 
      CD4(-) CD8(+) cells are the least permissive. However, the least susceptible 
      CD4(-) CD8(+) DC subset was the best interleukin-12 producer in response to 
      infection. Infection did not induce in any DC subset production of the 
      proinflammatory cytokine gamma interferon and nitric oxide associated with the 
      induction of Th1 responses. The number of parasites phagocytosed by DCs was low, 
      no more than 3 organisms per cell, compared to more than 10 organisms per 
      macrophage. In infected DCs, the parasites are located in a parasitophorous 
      vacuole containing both major histocompatibility complex (MHC) class II and 
      lysosome-associated membrane protein 1 molecules, similar to their location in 
      the infected macrophage. The parasite-driven redistribution of MHC class II to 
      this compartment indicates that infected DCs should be able to present parasite 
      antigen.
FAU - Henri, Sandrine
AU  - Henri S
AD  - The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.
FAU - Curtis, Joan
AU  - Curtis J
FAU - Hochrein, Hubertus
AU  - Hochrein H
FAU - Vremec, David
AU  - Vremec D
FAU - Shortman, Ken
AU  - Shortman K
FAU - Handman, Emanuela
AU  - Handman E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 187348-17-0 (Interleukin-12)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - CD4 Antigens/*immunology
MH  - CD8 Antigens/*immunology
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology/parasitology
MH  - Interleukin-12/*biosynthesis
MH  - Intracellular Fluid/parasitology
MH  - Leishmania major/*immunology
MH  - Macrophages, Peritoneal/immunology/parasitology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide/biosynthesis
MH  - Spleen/cytology
PMC - PMC128092
EDAT- 2002/06/18 10:00
MHDA- 2002/07/31 10:01
PMCR- 2002/07/01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/07/31 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
PHST- 2002/07/01 00:00 [pmc-release]
AID - 1633 [pii]
AID - 10.1128/IAI.70.7.3874-3880.2002 [doi]
PST - ppublish
SO  - Infect Immun. 2002 Jul;70(7):3874-80. doi: 10.1128/IAI.70.7.3874-3880.2002.