PMID- 12067452
OWN - NLM
STAT- MEDLINE
DCOM- 20020722
LR  - 20200225
IS  - 1582-1838 (Print)
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Linking)
VI  - 5
IP  - 1
DP  - 2001 Jan-Mar
TI  - Localizing genes in Drosophila melanogaster polytene chromosomes by fluorescence 
      in situ hybridization.
PG  - 74-8
AB  - This paper describes a method for the identification of single copy genes in 
      Drosophila melanogaster polytene chromosomes, using fluorescence in situ 
      hybridization (FISH). We demonstrate the detection of white (w), a gene 
      previously mapped to 1-1.5 region of the linkage map, and to 3C2 region of the 
      cytogenetic map of X chromosome. Squash preparations of polytene chromosomes from 
      salivary glands dissected out from third instar larvae of Drosophila melanogaster 
      were denatured and subjected to hybridization with a digoxigenin labeled probe, 
      corresponding to mini-white gene. The preparations were then washed and incubated 
      with anti-digoxigenin-fluorescein antibodies. After removal of the 
      nonspecifically bound antibodies, the polytene chromosomes were counterstained 
      with propidium iodide. Fluorescence microscopy revealed white locus in the X 
      chromosome in a subterminal location, in agreement with the above mentioned maps. 
      The protocol is efficient and adaptable for simultaneously multiple signal 
      detection.
FAU - Gavrila, L
AU  - Gavrila L
AD  - Department of Genetics, Faculty of Biology, University of Bucharest, CENTRAGEN, 
      1-3 Portocalelor, 76258, sect.6, Bucharest, Romania. gavrila@botanic.unibuc.ro
FAU - Ecovoiu, A A
AU  - Ecovoiu AA
FAU - Georgescu, L M
AU  - Georgescu LM
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (w protein, Drosophila)
SB  - IM
MH  - *ATP-Binding Cassette Transporters
MH  - Animals
MH  - Chromosomes/*genetics
MH  - Drosophila Proteins/genetics
MH  - Drosophila melanogaster/*genetics/growth & development
MH  - Eye Proteins/genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - *Physical Chromosome Mapping
PMC - PMC6737751
EDAT- 2002/06/18 10:00
MHDA- 2002/07/23 10:01
PMCR- 2001/01/01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/07/23 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 005.001.06 [pii]
AID - JCMM74 [pii]
AID - 10.1111/j.1582-4934.2001.tb00139.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2001 Jan-Mar;5(1):74-8. doi: 10.1111/j.1582-4934.2001.tb00139.x.