PMID- 12067840
OWN - NLM
STAT- MEDLINE
DCOM- 20020710
LR  - 20161124
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 283
IP  - 1
DP  - 2002 Jul
TI  - Effects of caloric restriction on mitochondrial function and gene transcripts in 
      rat muscle.
PG  - E38-43
AB  - Rodent skeletal muscle mitochondrial DNA has been shown to be a potential site of 
      oxidative damage during aging. Caloric restriction (CR) is reported to reduce 
      oxidative stress and prolong life expectancy in rodents. Gene expression 
      profiling and measurement of mitochondrial ATP production capacity were performed 
      in skeletal muscle of male rats after feeding them either a control diet or 
      calorie-restricted diet (60% of control diet) for 36 wk to determine the 
      potential mechanism of the beneficial effects of CR. CR enhanced the transcripts 
      of genes involved in reactive oxygen free radical scavenging function, tissue 
      development, and energy metabolism while decreasing expression of those genes 
      involved in signal transduction, stress response, and structural and contractile 
      proteins. Real-time PCR measurements confirmed the changes in transcript levels 
      of cytochrome-c oxidase III, superoxide dismutase (SOD)1, and SOD2 that were 
      noted by the microarray approach. Mitochondrial ATP production and citrate 
      synthase were unaltered by the dietary changes. We conclude that CR alters 
      transcript levels of several genes in skeletal muscle and that mitochondrial 
      function in skeletal muscle remains unaltered by the dietary intervention. 
      Alterations in transcripts of many genes involved in reactive oxygen scavenging 
      function may contribute to the increase in longevity reported with CR.
FAU - Sreekumar, R
AU  - Sreekumar R
AD  - Endocrinology Division, Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - Unnikrishnan, J
AU  - Unnikrishnan J
FAU - Fu, A
AU  - Fu A
FAU - Nygren, J
AU  - Nygren J
FAU - Short, K R
AU  - Short KR
FAU - Schimke, J
AU  - Schimke J
FAU - Barazzoni, R
AU  - Barazzoni R
FAU - Nair, K Sreekumaran
AU  - Nair KS
LA  - eng
GR  - R01 AG-09531/AG/NIA NIH HHS/United States
GR  - T32-DK-07352/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Carrier Proteins)
RN  - 0 (Ion Channels)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Uncoupling Protein 2)
RN  - 0 (Uncoupling Protein 3)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.15.1.1 (Sod1 protein, rat)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
RN  - EC 1.15.1.1 (superoxide dismutase 2)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Blotting, Northern
MH  - Body Weight/physiology
MH  - Carrier Proteins/genetics
MH  - Citrate (si)-Synthase/metabolism
MH  - Electron Transport Complex IV/genetics
MH  - Energy Intake/*physiology
MH  - Gene Expression Profiling
MH  - Ion Channels
MH  - Male
MH  - *Membrane Transport Proteins
MH  - Mitochondria/*genetics/*metabolism
MH  - *Mitochondrial Proteins
MH  - Muscle, Skeletal/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction
MH  - Proteins/genetics
MH  - RNA, Messenger/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/genetics
MH  - Superoxide Dismutase-1
MH  - Uncoupling Protein 2
MH  - Uncoupling Protein 3
EDAT- 2002/06/18 10:00
MHDA- 2002/07/12 10:01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/07/12 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
AID - 10.1152/ajpendo.00387.2001 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2002 Jul;283(1):E38-43. doi: 
      10.1152/ajpendo.00387.2001.