PMID- 12068008
OWN - NLM
STAT- MEDLINE
DCOM- 20020923
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 34
DP  - 2002 Aug 23
TI  - Activation of monocyte cyclooxygenase-2 gene expression by human herpesvirus 6. 
      Role for cyclic AMP-responsive element-binding protein and activator protein-1.
PG  - 30665-74
AB  - Prostaglandin E(2) (PGE(2)) is an arachidonic acid metabolite mainly produced by 
      activated monocytes/macrophages (Mo/Mphi) that display broad immunomodulatory 
      activities. Several viruses capable of infecting Mo/Mphi modulate PGE(2) 
      synthesis in a way that favors the infection processes and the spread of virions. 
      In the present work, we studied the effect of human herpesvirus 6 (HHV-6) 
      infection of Mo/Mphi on PGE(2) synthesis. Our results indicate that HHV-6 induces 
      COX-2 gene expression and PGE(2) synthesis within a few hours of infection. We 
      mapped the different promoter elements associated with COX-2 gene activation by 
      HHV-6 to two cis-acting elements: a cyclic AMP-responsive element and an 
      activator protein-1 element. HHV-6 immediate-early protein 2 was identified as a 
      modulator of COX-2 gene expression in Mo/Mphi. Finally, addition of PGE(2) to 
      HHV-6-infected peripheral blood mononuclear cells cultures was found to increase 
      significantly viral replication. Overall, these results further contribute to the 
      immunomodulatory properties of HHV-6 and highlight a potential role for 
      eicosanoids in the replication process of this virus.
FAU - Janelle, Marie-Eve
AU  - Janelle ME
AD  - Laboratory of Virology and Laboratory of Viral Immunology, Rheumatology, and 
      Immunology Research Center, CHUL Research Center and Faculty of Medicine, Laval 
      University, Quebec G1V 4G2, Canada.
FAU - Gravel, Annie
AU  - Gravel A
FAU - Gosselin, Jean
AU  - Gosselin J
FAU - Tremblay, Michel J
AU  - Tremblay MJ
FAU - Flamand, Louis
AU  - Flamand L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020614
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Proteins)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Cells, Cultured
MH  - Cyclic AMP Response Element-Binding Protein/*physiology
MH  - Cyclooxygenase 2
MH  - DNA-Binding Proteins/physiology
MH  - Dinoprostone/*biosynthesis/pharmacology
MH  - *Gene Expression Regulation, Enzymologic
MH  - Herpesvirus 6, Human/drug effects/*physiology
MH  - Humans
MH  - Isoenzymes/*genetics
MH  - Membrane Proteins
MH  - Monocytes/metabolism
MH  - NFATC Transcription Factors
MH  - *Nuclear Proteins
MH  - Promoter Regions, Genetic
MH  - Prostaglandin-Endoperoxide Synthases/*genetics
MH  - T-Lymphocytes/immunology
MH  - Transcription Factor AP-1/*physiology
MH  - Transcription Factors/physiology
MH  - Transcriptional Activation
MH  - Virus Replication
EDAT- 2002/06/18 10:00
MHDA- 2002/09/24 06:00
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/09/24 06:00 [medline]
PHST- 2002/06/18 10:00 [entrez]
AID - S0021-9258(20)70144-4 [pii]
AID - 10.1074/jbc.M203041200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Aug 23;277(34):30665-74. doi: 10.1074/jbc.M203041200. Epub 2002 
      Jun 14.