PMID- 12068077
OWN - NLM
STAT- MEDLINE
DCOM- 20020712
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 81
IP  - 6
DP  - 2002 Jun
TI  - Further characterization of the molecular interaction between PSD-95 and NMDA 
      receptors: the effect of the NR1 splice variant and evidence for modulation of 
      channel gating.
PG  - 1298-307
AB  - Coexpression of PSD-95(c-Myc) with NR1-1a/NR2A NMDA receptors in human embryonic 
      kidney (HEK) 293 cells resulted in a decrease in efficacy for the glycine 
      stimulation of [3 H]MK801 binding similar to that previously described for 
      l-glutamate. The inhibition constants (K (I) s) for the binding of l-glutamate 
      and glycine to NR1-1a/NR2A determined by [3 H]CGP 39653 and [3 H]MDL 105 519 
      displacement assays, respectively, were not significantly different between 
      NR1-1a/NR2A receptors coexpressed +/- PSD-95(c-Myc). The increased EC(50) for 
      l-glutamate enhancement of [3 H]MK801 binding was also found for NR1-2a/NR2A and 
      NR1-4b/NRA receptors thus the altered EC(50) is not dependent on the N1, C1 or C2 
      exon of the NR1 subunit. The NR1-4b but not the NR1-1a subunit was expressed 
      efficiently at the cell surface in the absence of NR2 subunits. Total NR1-4b and 
      NR1-4b/NR2A expression was enhanced by PSD-95(c-Myc) but whole cell enzyme-linked 
      immunoadsorbent assays (ELISAs) showed that this increase was not due to 
      increased expression at the cell surface. It is suggested that PSD-95(c-Myc) has 
      a dual effect on NMDA receptors expressed in mammalian cells, a reduction in 
      channel gating and an enhanced expression of NMDA receptor subunits containing 
      C-terminal E(T/S)XV PSD-95 binding motifs.
FAU - Rutter, A Richard
AU  - Rutter AR
AD  - Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, 
      University of London, London, UK.
FAU - Freeman, Fiona M
AU  - Freeman FM
FAU - Stephenson, F Anne
AU  - Stephenson FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (DNA, Recombinant)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (postsynaptic density proteins)
RN  - 132472-31-2 (CGP 39653)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/*analogs & derivatives/metabolism
MH  - Binding, Competitive
MH  - Cell Line
MH  - *DNA, Recombinant
MH  - Dizocilpine Maleate/metabolism
MH  - Excitatory Amino Acid Antagonists/metabolism
MH  - Genetic Variation/*physiology
MH  - Glutamic Acid/metabolism
MH  - Glycine/pharmacology
MH  - Humans
MH  - Ion Channel Gating/*physiology
MH  - Nerve Tissue Proteins/pharmacology/*physiology
MH  - Prosencephalon/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/genetics/metabolism/*physiology
EDAT- 2002/06/18 10:00
MHDA- 2002/07/13 10:01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/07/13 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
AID - 10.1046/j.1471-4159.2002.00923.x [doi]
PST - ppublish
SO  - J Neurochem. 2002 Jun;81(6):1298-307. doi: 10.1046/j.1471-4159.2002.00923.x.