PMID- 12068082
OWN - NLM
STAT- MEDLINE
DCOM- 20020712
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 81
IP  - 6
DP  - 2002 Jun
TI  - Profound molecular changes following hippocampal slice preparation: loss of AMPA 
      receptor subunits and uncoupled mRNA/protein expression.
PG  - 1348-60
AB  - The acute hippocampal slice preparation is a convenient, in vitro model widely 
      used to study the biological basis of synaptic plasticity. Although slices may 
      preserve their electrophysiological properties for several hours, profound 
      molecular changes in response to the injury caused by the slicing procedure are 
      likely to occur. To determine the magnitude and duration of these changes we 
      examined the post-slicing expression kinetics of three classes of genes known to 
      be implicated in long-term synaptic plasticity: glutamate AMPA receptors (GluR), 
      transcription factors and neurotrophins. Slicing resulted in a striking loss of 
      GluR1 and GluR3, but not of GluR2 proteins suggesting that rapid changes in the 
      composition of major neurotransmitter receptors may occur. Slicing caused a 
      significant induction of the transcription factors c-fos, zif268, CCAAT enhancer 
      binding protein (C/EBP ) beta and delta mRNAs and of the neurotrophin 
      brain-derived neurothophic factor (BDNF ) mRNA. In contrast, there was no 
      augmentation, and sometimes a decline, in the levels of the corresponding 
      proteins. These data reveal that significant discrepancies exist between the 
      slice preparation and the intact hippocampus in terms of the metabolism of 
      molecular components known to be involved in synaptic plasticity.
FAU - Taubenfeld, Stephen M
AU  - Taubenfeld SM
AD  - Department of Physiology and Biophysics, Mount Sinai School of Medicine, New 
      York, New York 10029, USA.
FAU - Stevens, Kimberly A
AU  - Stevens KA
FAU - Pollonini, Gabriella
AU  - Pollonini G
FAU - Ruggiero, Jason
AU  - Ruggiero J
FAU - Alberini, Cristina M
AU  - Alberini CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Transcription Factors)
RN  - 0 (glutamate receptor ionotropic, AMPA 3)
RN  - TFZ3H25BS1 (glutamate receptor ionotropic, AMPA 1)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Hippocampus/*metabolism
MH  - *Histological Techniques
MH  - In Vitro Techniques
MH  - Neurotrophin 3/metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptors, AMPA/genetics/*metabolism
MH  - Transcription Factors/genetics/metabolism
EDAT- 2002/06/18 10:00
MHDA- 2002/07/13 10:01
CRDT- 2002/06/18 10:00
PHST- 2002/06/18 10:00 [pubmed]
PHST- 2002/07/13 10:01 [medline]
PHST- 2002/06/18 10:00 [entrez]
AID - 10.1046/j.1471-4159.2002.00936.x [doi]
PST - ppublish
SO  - J Neurochem. 2002 Jun;81(6):1348-60. doi: 10.1046/j.1471-4159.2002.00936.x.