PMID- 12070066
OWN - NLM
STAT- MEDLINE
DCOM- 20020726
LR  - 20071114
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 165
IP  - 12
DP  - 2002 Jun 15
TI  - Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus 
      calmette-guerin in rats.
PG  - 1640-6
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells that play a 
      central role in initiating the primary immune response. However, their role in 
      granulomatous inflammation has not been well studied. The aim of the present 
      study was to elucidate the role of DCs in granuloma formation. Using a rat model 
      of bacillus Calmette-Guerin (BCG)-elicited pulmonary granulomas, we investigated 
      the distribution of DCs in the granulomas by immunohistochemistry with a 
      rat-DC-specific monoclonal antibody, OX62. We found numerous large, pleiomorphic 
      OX62(+) cells accumulating at the borders of the pulmonary granulomas. The 
      OX62(+) cells isolated from the granulomatous lung showed intense surface 
      expression of major histocompatibility complex class II, B7-1, and B7-2, and a 
      lack of T cell- and monocyte/macrophage-specific markers. Their ultrastructural 
      morphology was characteristic of DCs. Functionally, they had potent capacity to 
      stimulate allogeneic T cells as well as purified protein derivative-specific 
      syngeneic T cells in the absence of exogenous peptides. Based on these findings, 
      the OX62(+) cells infiltrating the granulomas were considered to be DCs 
      expressing BCG-derived peptides. These results indicate that DCs contribute to 
      pulmonary granuloma formation elicited by BCG by means of their potent 
      antigen-presenting function, providing a novel insight into DC function in T 
      cell-mediated granulomatous immune responses.
FAU - Tsuchiya, Tomoyoshi
AU  - Tsuchiya T
AD  - Second Division, Department of Internal Medicine, Hamamatsu University School of 
      Medicine, Japan.
FAU - Chida, Kingo
AU  - Chida K
FAU - Suda, Takafumi
AU  - Suda T
FAU - Schneeberger, Eveline E
AU  - Schneeberger EE
FAU - Nakamura, Hirotoshi
AU  - Nakamura H
LA  - eng
GR  - HL36781/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (BCG Vaccine)
RN  - 0 (OX-62 antigen, rat)
SB  - IM
CIN - Am J Respir Crit Care Med. 2002 Jun 15;165(12):1577-8. PMID: 12070053
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity/immunology
MH  - Antigens, Differentiation/genetics/immunology/metabolism
MH  - BCG Vaccine/*administration & dosage
MH  - Cell Aggregation/immunology
MH  - Cell Differentiation/immunology
MH  - Cell Movement/immunology
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Disease Models, Animal
MH  - Granuloma/*immunology/metabolism
MH  - Immunohistochemistry
MH  - Immunomagnetic Separation
MH  - Lung/cytology/*immunology/metabolism
MH  - Male
MH  - Mycobacterium bovis/*immunology
MH  - Phenotype
MH  - Rats
MH  - Rats, Inbred Lew
MH  - T-Lymphocytes/cytology/immunology/metabolism
MH  - Time Factors
EDAT- 2002/06/19 10:00
MHDA- 2002/07/27 10:01
CRDT- 2002/06/19 10:00
PHST- 2002/06/19 10:00 [pubmed]
PHST- 2002/07/27 10:01 [medline]
PHST- 2002/06/19 10:00 [entrez]
AID - 10.1164/rccm.2110086 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2002 Jun 15;165(12):1640-6. doi: 10.1164/rccm.2110086.