PMID- 12070679 OWN - NLM STAT- MEDLINE DCOM- 20021212 LR - 20151119 IS - 0172-8172 (Print) IS - 0172-8172 (Linking) VI - 22 IP - 2 DP - 2002 Jun TI - Soluble HLA in saliva of patients with autoimmune rheumatic diseases. PG - 71-6 AB - OBJECTIVES: The limited number of studies addressing the presence of soluble human leukocyte antigen (HLA) in body fluids such as tears, urine, sweat, and saliva are restricted to healthy subjects. In this study, we applied solid-phase enzyme-linked immunoassay to quantify soluble HLA class I (sHLA-I) and class II (sHLA-II) molecules in saliva and in selected serum samples obtained from patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and Sjogren's syndrome (SS). RESULTS: While saliva from normal subjects (n=35) contained levels of sHLA-I that were undetectable (n=7) or ranged from 9 ng/ml to 70 ng/ml (30+/-3.7 ng/ml, n=28), sHLA-I among 38 patients with moderately active RA ( n=24) or SLE (n=14) were significantly elevated (222+/-81.4 ng/ml and 120+/-27.3 ng/ml, respectively, P<0.0001). Intriguingly, sHLA-I levels were even higher in patients whose disease was regarded as active. Specifically, sHLA-I averaged 683+/-189 ng/ml in patients with active RA (n=13) and 230+/-24.5 ng/ml in patients with active SLE (n=7). This was significantly higher than in patients with milder forms of these diseases (P<0.0001). All five subjects with SS had severe disease with high levels of sHLA-I (mean 486+/-86 ng/ml). The concentrations of saliva sHLA-II in the disease groups studied were comparable with levels found in normal controls. The sHLA-I in severe and mild states was investigated in purified serum and saliva from a patient with SS. While 44-kDa fragments of serum HLA-I were detectable in severe SS, they were undetectable in saliva HLA at any time; 35-37-kDa fragments of serum or saliva HLA-I were detected during both mild and severe disease. Interestingly, the 39-kDa fragment was detected in both body fluids during severe but not mild forms of SS. Similarly, the isoforms with the molecular masses 39 kDa and 44 kDa were mainly identified in the purified material obtained from serum of SLE patients. CONCLUSIONS: Collectively, our data suggest that the measurement of soluble HLA in body fluids can be of both diagnostic and prognostic value in the assessment of patients with autoimmune rheumatic disorders. The mechanism by which sHLA enters saliva is unclear, but they probably are not acquired from serum. FAU - Adamashvili, Irena AU - Adamashvili I AD - Louisiana State University Health Sciences Center, Department of Surgery, P.O. Box 33932, Shreveport 71130-3932, USA. FAU - Pressly, Thomas AU - Pressly T FAU - Gebel, Howard AU - Gebel H FAU - Milford, Edgar AU - Milford E FAU - Wolf, Robert AU - Wolf R FAU - Mancini, Mary AU - Mancini M FAU - Sittg, Kevin AU - Sittg K FAU - Ghali, G E AU - Ghali GE FAU - Hall, Vicky AU - Hall V FAU - McDonald, John C AU - McDonald JC LA - eng PT - Journal Article DEP - 20020504 PL - Germany TA - Rheumatol Int JT - Rheumatology international JID - 8206885 RN - 0 (Biomarkers) RN - 0 (Histocompatibility Antigens Class I) RN - 0 (Histocompatibility Antigens Class II) SB - IM MH - Adult MH - Aged MH - Arthritis, Rheumatoid/*immunology MH - Biomarkers MH - Blotting, Western MH - Female MH - Histocompatibility Antigens Class I/*analysis MH - Histocompatibility Antigens Class II/*analysis MH - Histocompatibility Testing MH - Humans MH - Lupus Erythematosus, Systemic/*immunology MH - Male MH - Middle Aged MH - Reference Values MH - Saliva/*immunology EDAT- 2002/06/19 10:00 MHDA- 2002/12/13 04:00 CRDT- 2002/06/19 10:00 PHST- 2001/11/19 00:00 [received] PHST- 2002/03/10 00:00 [accepted] PHST- 2002/06/19 10:00 [pubmed] PHST- 2002/12/13 04:00 [medline] PHST- 2002/06/19 10:00 [entrez] AID - 10.1007/s00296-002-0173-3 [doi] PST - ppublish SO - Rheumatol Int. 2002 Jun;22(2):71-6. doi: 10.1007/s00296-002-0173-3. Epub 2002 May 4.