PMID- 12072201
OWN - NLM
STAT- MEDLINE
DCOM- 20020724
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 135
IP  - 1
DP  - 2002 May
TI  - Spectrum of genetic changes in gastro-esophageal cancer cell lines determined by 
      an integrated molecular cytogenetic approach.
PG  - 35-41
AB  - Adenocarcinomas arising around the gastro-esophageal junction (GEJ) are highly 
      malignant, and their incidence has risen rapidly in the last decades. Cell lines 
      are the basic in vitro system for functional and therapeutic studies in GEJ 
      tumors, but only a small number of cell lines are currently available, and none 
      of them has been fully karyotyped. We analyzed 5 GEJ tumor cell lines using a 
      combination of 24-color fluorescence in situ hybridization (FISH), comparative 
      genomic hybridization (CGH) and genomic microarrays. Using CGH we demonstrated 
      that these cell lines present imbalances similar to those we had previously 
      observed in primary GEJ tumors, namely gains on 1q, 7q, 8q, 17q, 19q, 20, and X, 
      and losses on 3p, 4, 5q, 9p, 18q, and 21. Multicolor FISH karyotyping revealed 
      multiple structural rearrangements involving chromosomes 1, 5, 6, 7, 8, 9, 13, 
      17, 18, and 22. Rearrangements of chromosome 8 involved 10 different chromosomes, 
      while rearrangements of chromosome 17 involved 5. Different rearrangements 
      resulted in imbalances of similar chromosome regions, suggesting that similar 
      genomic imbalances are constitutively important but are achieved through 
      different pathways. The use of a commercially available genomic array excluded 
      TOP2A (17q), and MYBL2, PTPT1, CSE1L, and ZNF217 (20q) as candidate genes for 
      frequently amplified areas on these chromosomes, and contributed to refining the 
      limits of chromosome regions involved in genomic imbalances.
FAU - Rosenberg, Carla
AU  - Rosenberg C
AD  - Laboratory of Cytochemistry and Cytometry, Department Molecular Cell Biology, 
      Leiden University Medical Center (LUMC), Leiden, The Netherlands. 
      c.rosenberg@lumc.nl
FAU - Geelen, Eric
AU  - Geelen E
FAU - IJszenga, Marije J
AU  - IJszenga MJ
FAU - Pearson, Peter
AU  - Pearson P
FAU - Tanke, Hans J
AU  - Tanke HJ
FAU - Dinjens, Winand N M
AU  - Dinjens WN
FAU - van Dekken, Herman
AU  - van Dekken H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Aneuploidy
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*genetics/ultrastructure
MH  - Esophageal Neoplasms/*genetics/pathology
MH  - Esophagogastric Junction/*pathology
MH  - Gene Amplification
MH  - Gene Expression Profiling
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Neoplasm Proteins/biosynthesis/genetics
MH  - *Nucleic Acid Hybridization
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Stomach Neoplasms/*genetics/pathology
MH  - Tumor Cells, Cultured
EDAT- 2002/06/20 10:00
MHDA- 2002/07/26 10:01
CRDT- 2002/06/20 10:00
PHST- 2002/06/20 10:00 [pubmed]
PHST- 2002/07/26 10:01 [medline]
PHST- 2002/06/20 10:00 [entrez]
AID - S0165460801006392 [pii]
AID - 10.1016/s0165-4608(01)00639-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 May;135(1):35-41. doi: 
      10.1016/s0165-4608(01)00639-2.