PMID- 12072237
OWN - NLM
STAT- MEDLINE
DCOM- 20020730
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 87
IP  - 3-4
DP  - 2002 Sep 10
TI  - Modulating immune responses with dendritic cells: an attainable goal in 
      veterinary medicine?
PG  - 215-21
AB  - Dendritic cells (DCs) are antigen presenting cells that potently modulate immune 
      responses with varying outcomes depending on the DC sub-population involved. To 
      understand how DC sub-types arise, it is necessary to determine which factors 
      influence their differentiation. At least three major sub-populations of DCs have 
      been described in mice: CD4+/CD8- "myeloid" DCs, CD4-/CD8+ "lymphoid" DCs and 
      Langerhans cell-derived DCs. Whilst somewhat comparable populations have been 
      described in man, in most other species very little is known. The identification 
      of cytokines which stimulate proliferation of DC precursors, and the observation 
      that the cytokine environment influences the phenotype and the function of the 
      DCs that subsequently develop, has provided a useful tool for evaluating these 
      rare cells. We describe the influence of cytokines on the phenotype of DCs 
      generated in the rat. Using bone marrow cells as the source of precursors we 
      generated "myeloid-type" DCs from the adherent population using 
      granulocyte-macrophage colony stimulating factor (GM-CSF), IL-4 and Flt-3L or 
      "lymphoid-type" DCs from the non-adherent population using cytokines which 
      included IL-7, IL-3, SCF and TNFalpha. In order to facilitate similar approaches 
      to the study of equine DCs we have identified the nucleotide sequence encoding 
      GM-CSF from the m-RNA of equine PBMC stimulated with Concanavalin A, amplified 
      the cDNA by PCR and cloned it in eukaryotic and prokaryotic expression vectors. 
      We report on the structure and function of this molecule.
FAU - Vecchione, Anna
AU  - Vecchione A
AD  - Department of Pathology and Infectious Diseases, Royal Veterinary College, London 
      NW1 0TU, UK. avecchione@rvc.ac.uk
FAU - Catchpole, Brian
AU  - Catchpole B
FAU - D'Mello, Felicity
AU  - D'Mello F
FAU - Kanellos, Theo
AU  - Kanellos T
FAU - Hamblin, Anne
AU  - Hamblin A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Integrin alphaXbeta2)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*physiology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Horses
MH  - Integrin alphaXbeta2/analysis
MH  - Rats
MH  - *Veterinary Medicine
EDAT- 2002/06/20 10:00
MHDA- 2002/07/31 10:01
CRDT- 2002/06/20 10:00
PHST- 2002/06/20 10:00 [pubmed]
PHST- 2002/07/31 10:01 [medline]
PHST- 2002/06/20 10:00 [entrez]
AID - S016524270200079X [pii]
AID - 10.1016/s0165-2427(02)00079-x [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2002 Sep 10;87(3-4):215-21. doi: 
      10.1016/s0165-2427(02)00079-x.