PMID- 12073149
OWN - NLM
STAT- MEDLINE
DCOM- 20020730
LR  - 20061115
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 54
IP  - 3
DP  - 2002 Jun
TI  - Molecular cloning and expression analysis of tumor necrosis factor alpha from a 
      marine fish reveal its constitutive expression and ubiquitous nature.
PG  - 200-7
AB  - The tumor necrosis factor alpha ( TNF alpha) gene from the marine fish, gilthead 
      seabream (Sparus aurata L.), has been isolated by RT-PCR using degenerate primers 
      designed against vertebrate TNF alpha conserved motifs and subsequent rapid 
      amplification of cDNA ends (RACE). The TNF alpha cDNA consists of a 142 bp 5' 
      untranslated region (5'UTR), a single open reading frame of 762 bp, which could 
      code for a 253 amino acid protein, and a 476-bp 3'UTR. The protein sequence 
      deduced from seabream TNF alpha gene shows a high degree of homology with the 
      Japanese flounder TNF alpha (65.6% identity and 78.9% similarity) and, more 
      important, it is more homologous to mammalian TNF alphas (41.1-48.6% similarity) 
      than to TNF betas (36.0-43.5% similarity). The prediction of a transmembrane 
      domain between residues 37 and 54 of seabream TNF alpha and the presence of a 
      conserved Thr-Leu sequence, which is associated with cleavage of the mouse TNF 
      alpha molecule, suggest that seabream TNF alpha exists in two forms, a 
      membrane-bound and a soluble form. RT-PCR shows that the seabream TNF alpha 
      messenger was widely and constitutively accumulated. Lastly, stimuli known to 
      up-regulate seabream IL-1 beta, lipopolysaccharide and lymphocyte-derived 
      macrophage-activating factor, failed to up-regulate TNF alpha in cultured 
      macrophages. The putative role of three AU-rich endotoxin-responsive motifs 
      (AREs) of seabream TNF alpha mRNA, found within two phylogenetically conserved 
      protein binding regions, is discussed.
FAU - Garcia-Castillo, Jesus
AU  - Garcia-Castillo J
AD  - Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 
      Murcia, Spain.
FAU - Pelegrin, Pablo
AU  - Pelegrin P
FAU - Mulero, Victoriano
AU  - Mulero V
FAU - Meseguer, Jose
AU  - Meseguer J
LA  - eng
SI  - GENBANK/AJ413189
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020417
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Fish Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Fish Proteins/*biosynthesis/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - Sea Bream/*genetics/*immunology/metabolism
MH  - Sequence Alignment
MH  - Tissue Distribution
MH  - Transcription, Genetic
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/*genetics
EDAT- 2002/06/20 10:00
MHDA- 2002/07/31 10:01
CRDT- 2002/06/20 10:00
PHST- 2001/10/24 00:00 [received]
PHST- 2002/02/27 00:00 [revised]
PHST- 2002/06/20 10:00 [pubmed]
PHST- 2002/07/31 10:01 [medline]
PHST- 2002/06/20 10:00 [entrez]
AID - 10.1007/s00251-002-0451-y [doi]
PST - ppublish
SO  - Immunogenetics. 2002 Jun;54(3):200-7. doi: 10.1007/s00251-002-0451-y. Epub 2002 
      Apr 17.