PMID- 12073827
OWN - NLM
STAT- MEDLINE
DCOM- 20021218
LR  - 20061115
IS  - 1217-8950 (Print)
IS  - 1217-8950 (Linking)
VI  - 49
IP  - 1
DP  - 2002
TI  - Fluorescence in situ hybridization (FISH) in the molecular cytogenetics of 
      cancer.
PG  - 69-80
AB  - In this review, we discuss the developments of fluorescence in situ hybridization 
      (FISH) and place them in the context of their applications in cancer research. 
      These methods are not only very useful for the causal analysis of the development 
      and spread of certain tumors, they are also efficient tools for tumor diagnosis. 
      Although a review of all of the literature in this field is not possible here, 
      many of the major contributions are summarized along with recent work from our 
      laboratory. Our group contributes to the goal of functional identification of 
      tumor growth antagonizing genes. FISH and molecular analyses have shown that the 
      short arm of human chromosome 3 is frequently deleted in kidney, lung, breast, 
      uterus, testis and ovary carcinomas. Deletion-mapping studies have outlined 
      several separate deletion prone regions in different tumors, namely 3pter-p25, 
      p22-p21.3, p21.1-p14 and p14-p12, which may contain putative tumor suppressor 
      genes (TSGs). Candidate suppressor genes isolated from frequently deleted regions 
      need to be assayed for possible tumor-antagonizing ability by functional tests. 
      We have developed a functional test system, the microcell hybrid (MCH) based 
      "elimination test" (Et). The Et is based on the introduction of a single human 
      chromosome into tumor cells of human or murine origin, via microcell fusion. The 
      MCHs were analyzed by FISH painting and PCR for the elimination or retention of 
      specific human chromosome 3 (chr. 3) regions after one or several passages in 
      severe combined immunedeficient (SCID) mice. We have defined a common eliminated 
      region (CER) on chr. 3p21.3. CER is approximately 1 megabase (Mb) in size. We 
      have covered this region with PACs (bacteriophage PI based artificial chromosome) 
      and used FISH mapping for localization and ordering PACs and cosmids on the 
      chromosome 3 and high-resolution free chromatin/DNA fiber FISH to orient the PAC 
      contig, to measure the lengths of PACs, and to establish their order. Activation 
      of cellular oncogene by chromosomal tanslocation, which brings an oncogene under 
      the influence of a highly active chromosome region, appears to play a pivotal 
      role in the genesis of certain hematopoetic and lymphoid tumors. We have detected 
      specific chromosomal translocations by FISH painting in mouse plamacytoma (MPC), 
      human Burkitt lymphoma (BL) other B-cell derived tumors. We have showed in a 
      murine sarcoma derived line (SEWA) that FISH can be also be used for detection of 
      amplified oncogene (c-myc) and the linked locus (pvt-1). We have also applied the 
      FISH technique for visualization of integrated and episomal Epstein-Barr virus 
      (EBV) genomes and EBV transcripts in EBV-carrying B-cell derived human cell 
      lines.
FAU - Szeles, Anna
AU  - Szeles A
AD  - Microbiology and Tumor Biology Center, Karolinska Institute, Box 280, 17177 
      Stockholm, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Hungary
TA  - Acta Microbiol Immunol Hung
JT  - Acta microbiologica et immunologica Hungarica
JID - 9434021
SB  - IM
MH  - *Cytogenetic Analysis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Neoplasms/*diagnosis/*genetics
RF  - 31
EDAT- 2002/06/21 10:00
MHDA- 2002/12/19 04:00
CRDT- 2002/06/21 10:00
PHST- 2002/06/21 10:00 [pubmed]
PHST- 2002/12/19 04:00 [medline]
PHST- 2002/06/21 10:00 [entrez]
AID - 10.1556/AMicr.49.2002.1.7 [doi]
PST - ppublish
SO  - Acta Microbiol Immunol Hung. 2002;49(1):69-80. doi: 10.1556/AMicr.49.2002.1.7.