PMID- 12074588
OWN - NLM
STAT- MEDLINE
DCOM- 20020730
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 294
IP  - 5
DP  - 2002 Jun 28
TI  - Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor 
      protein with the TrkB tyrosine kinase receptor.
PG  - 1087-92
AB  - Brain-derived neurotrophic factor (BDNF) binds to and activates the TrkB tyrosine 
      kinase receptor to regulate cell differentiation, survival, and neural plasticity 
      in the nervous system. However, the identities of the downstream signaling 
      proteins involved in this process remain unclear. Using a yeast two-hybrid screen 
      with the intracellular domain (ICD-TrkB) of the TrkB BDNF receptor, we identified 
      the Nck2 adaptor protein as a novel interaction partner of the active form of 
      TrkB. Additionally, we identified three tyrosines in ICD-TrkB (Y694, Y695, and 
      Y771) that are crucial for this interaction. Similar results were obtained for 
      Nck1, an Nck2 homolog. We also found that TrkB could be co-precipitated with 
      GST-Nck2 recombinant protein or anti-Nck antibody in BDNF-activated cortical 
      neurons. These results suggest that BDNF stimulation promotes interaction of Ncks 
      with TrkB in cortical neurons.
FAU - Suzuki, Shingo
AU  - Suzuki S
AD  - Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 
      565-0871, Japan.
FAU - Mizutani, Masaharu
AU  - Mizutani M
FAU - Suzuki, Kenji
AU  - Suzuki K
FAU - Yamada, Masashi
AU  - Yamada M
FAU - Kojima, Masami
AU  - Kojima M
FAU - Hatanaka, Hiroshi
AU  - Hatanaka H
FAU - Koizumi, Shinichi
AU  - Koizumi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/chemistry/metabolism
MH  - Nerve Tissue Proteins/analysis
MH  - Neurons/drug effects/*metabolism
MH  - Rats
MH  - Receptor, trkB/chemistry/*metabolism
MH  - Two-Hybrid System Techniques
MH  - Tyrosine/analysis
EDAT- 2002/06/21 10:00
MHDA- 2002/07/31 10:01
CRDT- 2002/06/21 10:00
PHST- 2002/06/21 10:00 [pubmed]
PHST- 2002/07/31 10:01 [medline]
PHST- 2002/06/21 10:00 [entrez]
AID - S0006-291X(02)00606-X [pii]
AID - 10.1016/S0006-291X(02)00606-X [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Jun 28;294(5):1087-92. doi: 
      10.1016/S0006-291X(02)00606-X.