PMID- 12076289
OWN - NLM
STAT- MEDLINE
DCOM- 20020712
LR  - 20220330
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 42
IP  - 4
DP  - 2002 Apr
TI  - Collection of autologous monocytes for dendritic cell vaccination therapy in 
      metastatic melanoma patients.
PG  - 428-32
AB  - BACKGROUND: Dendritic cells (DCs) for immunotherapy of malignant melanoma can be 
      generated from partially enriched monocytes prepared from PBMNCs. The feasibility 
      of a single steady-state leukapheresis procedure to enrich monocytes for a 
      complete vaccination series with up to 10 vaccinations was investigated. STUDY 
      DESIGN AND METHODS: Thirty-eight patients (27 males and 11 females) with 
      metastatic melanoma were enrolled in the study. All leukapheresis procedures were 
      performed by a continuous flow method (Spectra, Cobe BCT) with a standard MNC 
      program. RESULTS: An average of 11.7 L (range, 8-14 L) of whole blood was 
      processed within 197.3 +/- 23.7 minutes, and a mean of 13.5 +/- 5.7 x 109 WBCs in 
      a final volume of 191.0 +/- 24.2 mL was collected. The MNC purity in the 
      apheresis component was 81.5 +/- 15.1 percent, from which 29.8 +/- 14.7 percent 
      were monocytes. Thus, 11.0 +/- 5.0 x 109 MNCs and 3.2 +/- 2.0 x 109 monocytes 
      were collected per procedure. Linear regression analysis revealed a high 
      correlation between the absolute number of monocytes in peripheral blood before 
      the apheresis procedure and the number of monocytes in the collected component 
      (r=0.74, p < 0.0001). For the generation of DCs, 1.6 +/- 0.8 x 109 MNCs were 
      plated into culture dishes; 3.2 +/- 1.8 percent of the cultured cells matured to 
      DCs, which resulted in 56.5 +/- 49.4 x 106 DCs (range, 6.3-178) per patient for 
      the complete vaccination series. CONCLUSION: A target dose of monocytes for the 
      complete vaccination series could be obtained by a single convenient, safe, 
      steady-state leukapheresis procedure in each patient without the need for G-CSF 
      mobilization. The absolute number of monocytes in peripheral blood before the 
      apheresis procedure is the best predictive variable for the yield of monocytes in 
      the apheresis component.
FAU - Nguyen, Xuan Duc
AU  - Nguyen XD
AD  - Institute of Transfusion Medicine and Immunology, Red Cross Blood Service of 
      Baden-Wurttemberg, Faculty of Clinical Medicine Mannheim, Germany. 
      x.nguyen@blutspende.de
FAU - Eichler, Hermann
AU  - Eichler H
FAU - Sucker, Antje
AU  - Sucker A
FAU - Hofmann, Udo
AU  - Hofmann U
FAU - Schadendorf, Dirk
AU  - Schadendorf D
FAU - Kluter, Harald
AU  - Kluter H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Humans
MH  - *Immunotherapy
MH  - Leukapheresis
MH  - Leukocyte Count
MH  - Male
MH  - Melanoma/blood/*therapy
MH  - Middle Aged
MH  - Monocytes/*immunology
MH  - Platelet Count
EDAT- 2002/06/22 10:00
MHDA- 2002/07/13 10:01
CRDT- 2002/06/22 10:00
PHST- 2002/06/22 10:00 [pubmed]
PHST- 2002/07/13 10:01 [medline]
PHST- 2002/06/22 10:00 [entrez]
AID - trf067 [pii]
AID - 10.1046/j.1525-1438.2002.00067.x [doi]
PST - ppublish
SO  - Transfusion. 2002 Apr;42(4):428-32. doi: 10.1046/j.1525-1438.2002.00067.x.