PMID- 12078203
OWN - NLM
STAT- MEDLINE
DCOM- 20021213
LR  - 20181130
IS  - 1000-7423 (Print)
IS  - 1000-7423 (Linking)
VI  - 16
IP  - 1
DP  - 1998
TI  - Effect of artemether on hexokinase, glucose phosphate isomerase and 
      phosphofructokinase of Schistosoma japonicum harbored in mice.
PG  - 25-8
AB  - AIM: To study the effect of artemether (Art) on hexokinase (HK), glucose 
      phosphate isomerase (GPI) and phosphofructokinase (PFK) of Schistosoma japonicum. 
      METHODS: Mice infected with schistosome cercariae for 4-5 wk were treated ig with 
      Art 100 or 300 mg.kg-1 and killed 24 h or 48 h after medication for collection of 
      schistosomes. The activities of HK, GPI and PFK of the worms were determined by 
      measuring the formation of NADPH or consumption of NADH. RESULTS: Worms from the 
      infected mice treated ig with Art at a single dose of 300 mg.kg-1 the inhibition 
      rates of HK activity of female and male worms were 33.7% and 13.7%, respectively 
      24 h after administration. Similar results were seen in GPI activity, but 48 h 
      after medication, the inhibition rate of GPI increased to 46.2% (female) and 
      32.9% (male), respectively. Worms from mice treated with Art 100 or 300 mg.kg-1, 
      the inhibitory effect on PFK in female worms was found much higher than that of 
      male worms the inhibition rates of PFK were 64.9%-71.0% in female worms and 
      16.3%-54.2% in male worms, respectively at 24 h and 48 h after treatment. 
      CONCLUSION: The results suggest that in the glycolytic pathway of schistosome PFK 
      might be one of the targets attacked by Art.
FAU - Xiao, S
AU  - Xiao S
AD  - Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, WHO 
      Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025.
FAU - You, J
AU  - You J
FAU - Guo, H
AU  - Guo H
FAU - Jiao, P
AU  - Jiao P
FAU - Mei, J
AU  - Mei J
FAU - Yao, M
AU  - Yao M
FAU - Feng, Z
AU  - Feng Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
JT  - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of 
      parasitology & parasitic diseases
JID - 8709992
RN  - 0 (Artemisinins)
RN  - 0 (Schistosomicides)
RN  - 0 (Sesquiterpenes)
RN  - C7D6T3H22J (Artemether)
RN  - EC 2.7.1 - (Phosphofructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
SB  - IM
MH  - Animals
MH  - Artemether
MH  - Artemisinins/*pharmacology
MH  - Female
MH  - Glucose-6-Phosphate Isomerase/*metabolism
MH  - Hexokinase/*metabolism
MH  - Male
MH  - Mice
MH  - Phosphofructokinases/*metabolism
MH  - Schistosoma japonicum/*enzymology
MH  - Schistosomicides/*pharmacology
MH  - Sesquiterpenes/*pharmacology
EDAT- 2002/06/25 10:00
MHDA- 2002/12/17 04:00
CRDT- 2002/06/25 10:00
PHST- 2002/06/25 10:00 [pubmed]
PHST- 2002/12/17 04:00 [medline]
PHST- 2002/06/25 10:00 [entrez]
PST - ppublish
SO  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1998;16(1):25-8.