PMID- 12080796
OWN - NLM
STAT- MEDLINE
DCOM- 20030124
LR  - 20061115
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 13
IP  - 4
DP  - 1999 Jul
TI  - [The separation and detection of the bioactive proteins in nerve regeneration 
      conditioned fluids].
PG  - 199-201
AB  - OBJECTIVE: To understand the biological activities of the nerve regeneration 
      conditioned fluid (NRCF). METHODS: Nerve regeneration chamber was made by using 
      silicone tube bridging distal and proximal ends of severed SD rat's sciatic 
      nerve. The biological activities of the proteins in NRCF, which were separated by 
      natural polyacrylamide gel electrophoresis (PAGE), were analysed by being 
      cocultured with excised neonatal dorsal root ganglia (DRG). RESULTS: Eight 
      separated protein bands of NRCF were observed between 67-669 ku in molecular 
      weight, and the protein bands between 232-440 ku showed strong neurotrophic and 
      chemotactic function. CONCLUSION: NRCF has the promoting effects on nerve 
      regeneration.
FAU - Li, Q F
AU  - Li QF
AD  - Department of Plastic Surgery, Shanghai 9th People's Hospital, Shanghai Second 
      Medical University, Shanghai, P. R. China 200011.
FAU - Xu, L P
AU  - Xu LP
FAU - Jing, N H
AU  - Jing NH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Ciliary Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Ciliary Neurotrophic Factor/*analysis/isolation & purification
MH  - Extracellular Space/*chemistry
MH  - Female
MH  - Male
MH  - *Nerve Regeneration
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/*physiopathology
EDAT- 2002/06/26 10:00
MHDA- 2003/01/25 04:00
CRDT- 2002/06/26 10:00
PHST- 2002/06/26 10:00 [pubmed]
PHST- 2003/01/25 04:00 [medline]
PHST- 2002/06/26 10:00 [entrez]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 1999 Jul;13(4):199-201.