PMID- 12082252
OWN - NLM
STAT- MEDLINE
DCOM- 20030717
LR  - 20181130
IS  - 1386-0291 (Print)
IS  - 1386-0291 (Linking)
VI  - 26
IP  - 3
DP  - 2002
TI  - Fluid shear stress induces the secretion of monocyte chemoattractant protein-1 in 
      cultured human umbilical vein endothelial cells.
PG  - 199-207
AB  - In this study we investigated the patterns of fluid shear stress on induction of 
      monocyte chemoattractant protein-1 (MCP-1) secretion in cultured human umbilical 
      vein endothelial cells (HUVECs). MCP-1 is a potent special chemoattractant, which 
      recruits monocytes into the sub-endothelium. This process is one of the early 
      events of atherosclerosis. We examined the pattern of fluid shear stress inducing 
      the secretion of MCP-1 in cultured HUVECs from the view of biomechanics. In our 
      experiments, HUVECs were subjected to controlled levels of shear stress (4, 10, 
      20 dyn/cm(2)) in a parallel plate flow chamber. MCP-1 in HUVECs of different 
      periods was measured by an immunohistochemistry method and digital image 
      analysis; MCP-1 in perfusion was measured by sandwich ELISA. The results 
      demonstrated the increase of MCP-1 synthesis and secretion by shear stress was 
      time- and force-dependent. The accumulated level of MCP-1 in HUVECs under lower 
      shear stress (4 dyn/cm(2)) for 4-5 hrs was 3-fold compared with that for static 
      cells. When the shear stress lasted to 6 hrs, the secretion of MCP-1 was reduced 
      to normal levels and could not be increased even when the shear stress lasted for 
      12 hours. 10 dyn/cm(2) had less effect on the secretion of MCP-1 compared with 4 
      dyn/cm(2). This research provides data for understanding the mechanism of the 
      contribution of hemodynamic forces to atherosclerosis.
FAU - Yu, Hongmei
AU  - Yu H
AD  - Biomedical Engineering Center, Beijing Polytechnic University, Beijing, 100022 
      China.
FAU - Zeng, Yanjun
AU  - Zeng Y
FAU - Hu, Jinlin
AU  - Hu J
FAU - Li, Caixia
AU  - Li C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Chemokine CCL2/biosynthesis/*metabolism
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Humans
MH  - Perfusion
MH  - Stress, Mechanical
MH  - Time Factors
MH  - Umbilical Veins
MH  - Up-Regulation
EDAT- 2002/06/26 10:00
MHDA- 2003/07/18 05:00
CRDT- 2002/06/26 10:00
PHST- 2002/06/26 10:00 [pubmed]
PHST- 2003/07/18 05:00 [medline]
PHST- 2002/06/26 10:00 [entrez]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 2002;26(3):199-207.