PMID- 12084877
OWN - NLM
STAT- MEDLINE
DCOM- 20020723
LR  - 20190514
IS  - 0028-3878 (Print)
IS  - 0028-3878 (Linking)
VI  - 58
IP  - 12
DP  - 2002 Jun 25
TI  - Differential expression of chemokines in inflammatory myopathies.
PG  - 1779-85
AB  - BACKGROUND: Chemokines represent a family of small-molecular-weight cytokines 
      that recruit and activate inflammatory cells in response to inflammation. 
      Invasion of cytotoxic memory T cells and macrophages in nonnecrotic muscle fibers 
      characterizes polymyositis and sporadic inclusion body myositis. Dermatomyositis 
      is a complement-mediated endotheliopathy. Elucidation of the mechanisms guiding 
      lymphocyte diapedesis and trafficking could lead to selective therapeutic 
      interventions. METHODS: Immunoblots and multistep immunofluorescence studies with 
      non-cross-reactive antibodies recognizing interleukin-8, monocyte chemoattractant 
      protein-1 (MCP-1), MCP-3, TARC (thymus and activation regulated cytokine), and 
      RANTES (regulated upon activation, normal T-cell expressed and secreted), using 
      appropriate positive and negative controls. In situ hybridization was used to 
      localize MCP-1 mRNA. RESULTS: MCP-1 protein was strongly expressed on T cells and 
      a subset of macrophages actively invading a proportion of the nonnecrotic muscle 
      fibers in polymyositis and inclusion body myositis alike. Capillaries and 
      arterioles in the vicinity of endomysial inflammatory foci were immunoreactive 
      for MCP-1, with faint or no expression in unaffected parts of the tissue. By 
      contrast, widespread and strong endothelial MCP-1 expression occurred on 
      perifascicular and perimysial endothelia in dermatomyositis, also at sites remote 
      from inflammatory infiltrates. In some control specimens, a subset of capillaries 
      also expressed MCP-1, possibly reflecting a role of this chemokine in normal 
      immune surveillance. MCP-1 mRNA was detected in scattered macrophages in each 
      inflammatory myopathy. All other chemokines were absent. CONCLUSION: Chemokines 
      are differentially expressed in the symptomatic stage of inflammatory myopathies. 
      MCP-1 plays a major role in the myocytotoxicity in polymyositis and inclusion 
      body myositis. MCP-1 may be induced by membranolytic attack complex binding to 
      endothelial cells in dermatomyositis.
FAU - De Bleecker, Jan L
AU  - De Bleecker JL
AD  - Department of Neurology, University Hospital, Gent, Belgium. 
      jan.debleecker@rug.ac.be
FAU - De Paepe, Boel
AU  - De Paepe B
FAU - Vanwalleghem, Iris E
AU  - Vanwalleghem IE
FAU - Schroder, J Michael
AU  - Schroder JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurology
JT  - Neurology
JID - 0401060
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
SB  - IM
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokines/*biosynthesis
MH  - Endothelium/immunology/metabolism/pathology
MH  - Humans
MH  - Lymph Nodes/immunology/metabolism/pathology
MH  - Muscle Fibers, Skeletal/immunology/metabolism/pathology
MH  - Myositis/immunology/*metabolism/*pathology
MH  - Thymus Gland/immunology/metabolism/pathology
MH  - Tumor Cells, Cultured
EDAT- 2002/06/27 10:00
MHDA- 2002/07/24 10:01
CRDT- 2002/06/27 10:00
PHST- 2002/06/27 10:00 [pubmed]
PHST- 2002/07/24 10:01 [medline]
PHST- 2002/06/27 10:00 [entrez]
AID - 10.1212/wnl.58.12.1779 [doi]
PST - ppublish
SO  - Neurology. 2002 Jun 25;58(12):1779-85. doi: 10.1212/wnl.58.12.1779.