PMID- 12085991
OWN - NLM
STAT- MEDLINE
DCOM- 20030730
LR  - 20191210
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 34
IP  - 2
DP  - 2002 May 31
TI  - Quantitative measurement of serum allergen-specific IgE on protein chip.
PG  - 152-8
AB  - Type I allergy is an immunoglobulin E (IgE)-mediated hypersensitivity disease 
      inflicting more than quarter of the world population. In order to identify 
      allergen sources, skin provocation test and IgE serology was performed using 
      allergen extracts. Such process identifies allergen-containing sources but cannot 
      identify the disease-eliciting allergenic molecules. Recently, microarray 
      technology has been developed for allergen-specific IgE detection using rolling 
      circle amplification. This study was carried out to evaluate protein chip 
      technology for the quantitative measurement and limits of sensitivity of multiple 
      allergen-specific IgE by an immunofluorescence assay. Significance of positive 
      calibrators was tested using purified human IgE. Dermatophagoides pteronyssinus 
      (Dp), egg white, milk, soybean, and wheat were used as allergens and human serum 
      albumin as negative control. Sensitivity and clinical efficacy of protein chip 
      were evaluated using allergy immune serum for Dp. The fluorescent intensities for 
      purified human IgE as calibrator were well correlated with the concentrations of 
      human IgE. Two-fold dilution of serum allowed an optimal reaction with Dp (1 
      mg/ml) at which serum Dp-specific IgE levels by protein chip were compatible with 
      those by UniCap. The sensitivity of protein chip in this study was found at level 
      of 1 IU/ml of IgE. Dp-specific IgE levels by protein chip correlated well with 
      those of UniCap by comparing 10 atopic dermatitis. Additional 18 sera were tested 
      for above multiple antigens other than Dp and significant results were obtained 
      for many antigens as well as Dp. These results indicated that spotting of 
      heterogeneous protein mixture on protein chip and the quantitative measurement of 
      serum allergen-specific IgE levels using immunofluorescence assay can be 
      successfully applied in the clinical laboratory for the diagnosis of allergy and 
      could be applied to diagnosis of autoimmune and infectious diseases
FAU - Kim, Tae-Eun
AU  - Kim TE
AD  - Molecular Immunology & Biochip Lab, Food Allergy Research Center, Food BioTech Co 
      Ltd, Seoul, Korea.
FAU - Park, Seok-Won
AU  - Park SW
FAU - Cho, Nam-Yun
AU  - Cho NY
FAU - Choi, Seung-Young
AU  - Choi SY
FAU - Yong, Tai-soon
AU  - Yong TS
FAU - Nahm, Baek-Hie
AU  - Nahm BH
FAU - Lee, Sangsun
AU  - Lee S
FAU - Noh, Geunwoong
AU  - Noh G
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology
MH  - Antibody Specificity
MH  - Dermatitis, Atopic/immunology
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Korea
MH  - Protein Array Analysis/*methods
MH  - Sensitivity and Specificity
EDAT- 2002/06/28 10:00
MHDA- 2003/07/31 05:00
CRDT- 2002/06/28 10:00
PHST- 2002/06/28 10:00 [pubmed]
PHST- 2003/07/31 05:00 [medline]
PHST- 2002/06/28 10:00 [entrez]
AID - 10.1038/emm.2002.22 [doi]
PST - ppublish
SO  - Exp Mol Med. 2002 May 31;34(2):152-8. doi: 10.1038/emm.2002.22.