PMID- 12087061
OWN - NLM
STAT- MEDLINE
DCOM- 20020723
LR  - 20131121
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 16
IP  - 9
DP  - 2002 Jul
TI  - Embryonic prostaglandin H synthase-2 (PHS-2) expression and benzo[a]pyrene 
      teratogenicity in PHS-2 knockout mice.
PG  - 1001-9
AB  - The developmental role of prostaglandin H synthase-2 (PHS-2), which converts 
      xenobiotics such as benzo[a]pyrene (B[a]P) to toxic free radical intermediates, 
      is poorly understood. In this study, we determined the embryonic expression and 
      teratological relevance of PHS-2 in pregnant CD-1 and B6/129S7 PHS-2 knockout 
      mice. Wild-type (+/+) B6/129S7 dams given B[a]P on gestational day (GD) 10 had 
      three times more fetal malformations than did +/- PHS-2-deficient dams (P<0.05). 
      GD 10-13 CD-1 embryos had high PHS-2 protein expression, and both + /+ and +/- GD 
      19 B6/129S7 fetuses had more B[a]P-initiated malformations and postpartum 
      lethality than did -/- littermates (P<0.05). Thus, embryonic PHS-2 is expressed 
      constitutively during organogenesis and contributes substantially to B[a]P 
      teratogenicity.
FAU - Parman, Toufan
AU  - Parman T
AD  - Faculty of Pharmacy and, Department of Pharmacology, University of Toronto, 
      Toronto, Ontario, Canada M5S 2S2.
FAU - Wells, Peter G
AU  - Wells PG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Isoenzymes)
RN  - 0 (Teratogens)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
SB  - IM
MH  - Abnormalities, Drug-Induced/enzymology/metabolism/mortality
MH  - Animals
MH  - Benzo(a)pyrene/pharmacokinetics/*toxicity
MH  - Biotransformation
MH  - Cyclooxygenase 2
MH  - Embryo, Mammalian/*enzymology
MH  - Embryonic and Fetal Development/drug effects
MH  - Female
MH  - Genotype
MH  - Isoenzymes/*genetics/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Morphogenesis
MH  - Pregnancy
MH  - Prostaglandin-Endoperoxide Synthases/*genetics/*metabolism
MH  - Species Specificity
MH  - Teratogens/pharmacokinetics/*toxicity
EDAT- 2002/06/28 10:00
MHDA- 2002/07/24 10:01
CRDT- 2002/06/28 10:00
PHST- 2002/06/28 10:00 [pubmed]
PHST- 2002/07/24 10:01 [medline]
PHST- 2002/06/28 10:00 [entrez]
AID - 16/9/1001 [pii]
AID - 10.1096/fj.01-0140com [doi]
PST - ppublish
SO  - FASEB J. 2002 Jul;16(9):1001-9. doi: 10.1096/fj.01-0140com.