PMID- 12087169
OWN - NLM
STAT- MEDLINE
DCOM- 20020719
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 30
IP  - 13
DP  - 2002 Jul 1
TI  - Internal ribosome entry site-mediated translation of Smad5 in vivo: requirement 
      for a nuclear event.
PG  - 2851-61
AB  - Smad5 is thought to relay signals of the bone morphogenetic protein pathway. The 
      5' untranslated region (5'UTR) of human Smad5 mRNA is long, has the potential to 
      form secondary structures and contains five AUG codons. Here we show that the 
      5'UTR of Smad5 contains an internal ribosome entry site (IRES) located within 100 
      nt of the 3' end of the 5'UTR. The Smad5 IRES was 4-8-fold more active than the 
      poliovirus IRES in C2C12 cells, which have osteoblastic differentiation ability, 
      but was 5-10-fold less active than the poliovirus IRES in 293T cells. When an in 
      vitro transcript of a dicistronic Smad5 IRES construct was transfected into C2C12 
      cells, the Smad5 IRES was not able to stimulate the translation of the downstream 
      cistron, although the cap-dependent translation of the upstream cistron was 
      efficient. In contrast, the poliovirus IRES in a dicistronic in vitro transcript 
      was able to stimulate the translation of the downstream cistron to a similar 
      extent as in the case of transfection of the corresponding dicistronic DNA 
      construct. These results suggest that Smad5 IRES activity displays cell 
      specificity and that some as yet unidentified nuclear event may be required for 
      efficient Smad5 IRES-driven translation initiation.
FAU - Shiroki, Kazuko
AU  - Shiroki K
AD  - Institute for Biomolecular Science, Gakushuin University, 1-5-1 Mejiro, 
      Toshima-ku, Tokyo 171-8588, Japan.
FAU - Ohsawa, Chieko
AU  - Ohsawa C
FAU - Sugi, Natuki
AU  - Sugi N
FAU - Wakiyama, Motoaki
AU  - Wakiyama M
FAU - Miura, Kin-ichiro
AU  - Miura K
FAU - Watanabe, Manabu
AU  - Watanabe M
FAU - Suzuki, Yutaka
AU  - Suzuki Y
FAU - Sugano, Sumio
AU  - Sugano S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SMAD5 protein, human)
RN  - 0 (Smad5 Protein)
RN  - 0 (Smad5 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 3T3 Cells
MH  - 5' Untranslated Regions/genetics/metabolism
MH  - Animals
MH  - Binding Sites/genetics
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Nucleus/genetics/metabolism
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Mice
MH  - Oocytes
MH  - Osteoblasts/cytology
MH  - Phosphoproteins/*genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Biosynthesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Ribosomes/*metabolism
MH  - Sequence Deletion
MH  - Smad5 Protein
MH  - Trans-Activators/*genetics
MH  - Transcription, Genetic
MH  - Xenopus
PMC - PMC117063
EDAT- 2002/06/28 10:00
MHDA- 2002/07/20 10:01
PMCR- 2002/07/01
CRDT- 2002/06/28 10:00
PHST- 2002/06/28 10:00 [pubmed]
PHST- 2002/07/20 10:01 [medline]
PHST- 2002/06/28 10:00 [entrez]
PHST- 2002/07/01 00:00 [pmc-release]
AID - gkf408 [pii]
AID - 10.1093/nar/gkf408 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2002 Jul 1;30(13):2851-61. doi: 10.1093/nar/gkf408.