PMID- 12089260
OWN - NLM
STAT- MEDLINE
DCOM- 20020826
LR  - 20231103
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 7
DP  - 2002 Jul
TI  - Real-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and 
      saliva.
PG  - 2445-51
AB  - A real-time quantitative PCR assay has been developed to measure human 
      herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 
      copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of 
      HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for 
      samples containing 10(2) DNA copies, 0.99 and 0.96% for samples containing 10(4) 
      copies, and 0.76 and 0.9% for samples containing 10(6) copies. Among 34 saliva 
      samples from healthy subjects, 26 were found to contain HHV-6 DNA (76.5%; median, 
      23,870 copies/ml), and following a single freeze-thaw cycle, 25 of the same 
      samples were found to be positive for HHV-6 DNA, although at a statistically 
      significantly lower concentration (median, 3,497 copies/ml). The assay enabled 
      detection of HHV-6 DNA in lymph node biopsies from patients with Hodgkin's 
      disease (HD) (13 of 37 patients [35.1%]), B-cell neoplasms (8 of 36 patients 
      [22.2%]), and T- or NK-cell neoplasms (3 of 13 patients [23.1%]), with 
      concentrations ranging from 100 to 864,640 HHV-6 copies per microg of DNA (HHV-6B 
      being found in every case except two). All HD patients infected with HHV-6 
      presented clinically with the nodular sclerosis subtype of HD. The real-time 
      quantitative PCR assay developed here was simple to perform and was sensitive 
      over a wide range of HHV-6 concentrations. It therefore appears to be of 
      potential value in clinical investigation or diagnosis of HHV-6 infection.
FAU - Collot, S
AU  - Collot S
AD  - Department of Virology, Limoges University Teaching Hospital, Limoges, France.
FAU - Petit, B
AU  - Petit B
FAU - Bordessoule, D
AU  - Bordessoule D
FAU - Alain, S
AU  - Alain S
FAU - Touati, M
AU  - Touati M
FAU - Denis, F
AU  - Denis F
FAU - Ranger-Rogez, S
AU  - Ranger-Rogez S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA, Viral/*analysis/*genetics
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/*genetics/*isolation & purification
MH  - Hodgkin Disease/virology
MH  - Humans
MH  - Leukemia, B-Cell/virology
MH  - Lymph Nodes/*virology
MH  - Lymphoma/virology
MH  - Lymphoma, B-Cell/virology
MH  - Lymphoma, T-Cell/virology
MH  - Lymphoproliferative Disorders/virology
MH  - Polymerase Chain Reaction/*methods/standards/statistics & numerical data
MH  - Reference Standards
MH  - Roseolovirus Infections/diagnosis/virology
MH  - Saliva/*virology
MH  - Sensitivity and Specificity
PMC - PMC120581
EDAT- 2002/06/29 10:00
MHDA- 2002/08/27 10:01
PMCR- 2002/07/01
CRDT- 2002/06/29 10:00
PHST- 2002/06/29 10:00 [pubmed]
PHST- 2002/08/27 10:01 [medline]
PHST- 2002/06/29 10:00 [entrez]
PHST- 2002/07/01 00:00 [pmc-release]
AID - 1439 [pii]
AID - 10.1128/JCM.40.7.2445-2451.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Jul;40(7):2445-51. doi: 10.1128/JCM.40.7.2445-2451.2002.