PMID- 12089275
OWN - NLM
STAT- MEDLINE
DCOM- 20020826
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 7
DP  - 2002 Jul
TI  - Detection of Epstein-Barr virus genomes in peripheral blood B cells from 
      solid-organ transplant recipients by fluorescence in situ hybridization.
PG  - 2533-44
AB  - Resolution of Epstein-Barr Virus (EBV) infection in pediatric solid-organ 
      transplant recipients often leads to an asymptomatic carrier state characterized 
      by a persistently elevated circulating EBV load that is 2 to 4 orders of 
      magnitude greater than the load typical of healthy latently infected individuals. 
      Elevated EBV loads in immunosuppressed individuals are associated with an 
      increased risk for development of posttransplant lymphoproliferative disease. We 
      have performed fluorescence in situ hybridization (FISH) studies with peripheral 
      blood B cells from carriers of persistent EBV loads in order to directly 
      quantitate the number of EBV genomes per infected cell. Patients were assigned to 
      two groups on the basis of the level of the persistent load (low-load carriers, 8 
      to 200 genomes/10(5) peripheral blood lymphocytes; high-load carriers, >200 
      genomes/10(5) peripheral blood lymphocytes). FISH analysis revealed that the 
      low-load carriers predominantly had circulating virus-infected cells harboring 
      one or two genome copies/cell. High-load carriers also had cells harboring one or 
      two genome copies/cell; in addition, however, they carried a distinct population 
      of cells with high numbers of viral genome copies. The increased viral loads 
      correlated with an increase in the frequency of cells containing high numbers of 
      viral genomes. We conclude that low-load carriers possess EBV-infected cells that 
      are in a state similar to normal latency, whereas high-load carriers possess two 
      populations of virus-positive B cells, one of which carries an increased number 
      of viral genomes per cell and is not typical of normal latency.
FAU - Rose, Camille
AU  - Rose C
AD  - Department of Infectious Diseases and Microbiology, Graduate School of Public 
      Health, Pittsburgh, Pennsylvania 15213, USA.
FAU - Green, Michael
AU  - Green M
FAU - Webber, Steven
AU  - Webber S
FAU - Kingsley, Lawrence
AU  - Kingsley L
FAU - Day, Roger
AU  - Day R
FAU - Watkins, Simon
AU  - Watkins S
FAU - Reyes, Jorges
AU  - Reyes J
FAU - Rowe, David
AU  - Rowe D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - B-Lymphocytes/*virology
MH  - Carrier State/virology
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/blood/genetics
MH  - Epstein-Barr Virus Infections/etiology/virology
MH  - Female
MH  - Genome, Viral
MH  - Herpesvirus 4, Human/*genetics/*isolation & purification/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/statistics & numerical data
MH  - Lymphoproliferative Disorders/etiology/virology
MH  - Male
MH  - Organ Transplantation/*adverse effects
MH  - Sensitivity and Specificity
MH  - Virus Latency
PMC - PMC120580
EDAT- 2002/06/29 10:00
MHDA- 2002/08/27 10:01
PMCR- 2002/07/01
CRDT- 2002/06/29 10:00
PHST- 2002/06/29 10:00 [pubmed]
PHST- 2002/08/27 10:01 [medline]
PHST- 2002/06/29 10:00 [entrez]
PHST- 2002/07/01 00:00 [pmc-release]
AID - 1436 [pii]
AID - 10.1128/JCM.40.7.2533-2544.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Jul;40(7):2533-44. doi: 10.1128/JCM.40.7.2533-2544.2002.