PMID- 12089350
OWN - NLM
STAT- MEDLINE
DCOM- 20030219
LR  - 20220408
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 16
IP  - 7
DP  - 2002 Jul
TI  - Characterization of a new upstream GnRH receptor promoter in human ovarian 
      granulosa-luteal cells.
PG  - 1552-64
AB  - GnRH has been implicated as an important local autocrine and paracrine factor in 
      regulating ovarian function. However, to date, the transcriptional regulation of 
      GnRH receptor (GnRHR) gene in human ovary remains poorly understood. Here we 
      report the characterization of a new upstream promoter for the GnRHR gene in 
      human granulosa-luteal cells. Using progressive deletion analysis, a region 
      between nucleotide -1300 and -1018 (relative to the translation start site) was 
      shown to exhibit the highest promoter activities in two immortalized human 
      granulosa-luteal cell lines, SVOG-4o and SVOG-4m. Two putative CCAAT/enhancer 
      binding protein (C/EBP) motifs and one GATA motif were identified within this 
      region. Mutational studies showed that these three motifs cooperated 
      synergistically to regulate GnRHR gene transcription in the granulosa cells but 
      not in other cell types including human ovarian carcinoma OVCAR-3, human 
      embryonic kidney-293 (HEK-293) and mouse pituitary gonadotrope-derived alphaT3-1 
      cells. Surprisingly, by competitive EMSAs, we found that an Oct-1 consensus 
      sequence was able to inhibit protein complex formation with the distal C/EBP 
      motif, suggesting a possible cross-talk between the Oct-1 transcription factor 
      and this C/EBP motif. Taken together, our results strongly indicate a role of the 
      C/EBP and GATA motifs in regulating GnRHR gene transcription in human 
      granulosa-luteal cells and further suggest that tissue-specific expression of 
      human GnRHR gene is mediated by differential promoter usage.
FAU - Cheng, Chi Keung
AU  - Cheng CK
AD  - Department of Obstetrics and Gynecology, University of British Columbia, 
      Vancouver, Canada V6H 3V5.
FAU - Yeung, Chung Man
AU  - Yeung CM
FAU - Chow, Billy K C
AU  - Chow BK
FAU - Leung, Peter C K
AU  - Leung PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (Receptors, LHRH)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Motifs
MH  - Base Sequence
MH  - Binding Sites
MH  - CCAAT-Enhancer-Binding Proteins/metabolism
MH  - Cell Line
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Electrophoretic Mobility Shift Assay
MH  - Erythroid-Specific DNA-Binding Factors
MH  - Female
MH  - Humans
MH  - Luteal Cells/*physiology
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Ovary/cytology
MH  - Promoter Regions, Genetic/*genetics
MH  - Receptors, LHRH/*genetics/metabolism
MH  - Transcription Factors/metabolism
MH  - Transcription Initiation Site
MH  - Transcription, Genetic
EDAT- 2002/06/29 10:00
MHDA- 2003/02/20 04:00
CRDT- 2002/06/29 10:00
PHST- 2002/06/29 10:00 [pubmed]
PHST- 2003/02/20 04:00 [medline]
PHST- 2002/06/29 10:00 [entrez]
AID - 10.1210/mend.16.7.0869 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2002 Jul;16(7):1552-64. doi: 10.1210/mend.16.7.0869.