PMID- 12091051
OWN - NLM
STAT- MEDLINE
DCOM- 20030304
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 105
IP  - 6
DP  - 2002 Mar 15
TI  - There is no valid evidence presented as to an impaired endothelial NO system in 
      the stroke-prone spontaneously hypertensive rats.
PG  - 507-11
AB  - Platelet reactivity in stroke-prone spontaneously hypertensive rats (SHRSP) and 
      normotensive Wistar-Kyoto rats (WKY) were compared. In vivo platelet reactivity 
      was tested by the He-Ne laser-induced thrombosis model. The number of laser 
      pulses needed to reach thrombotic occlusion of the targeted vessel was used as an 
      index of thrombogenicity. SHRSP rats needed significantly less number of 
      irradiation to reach occlusion than WKY rats (SHRSP vs. WKY, 5.1+/-0.3 vs. 
      8.1+/-0.6), indicating enhanced thrombotic response in SHRSP rats. Further, 
      acetylcholine administration significantly increased the number of laser pulses 
      until occlusion in WKY but not in SHRSP rats. This suggests an impaired 
      thrombotic reaction in acetylcholine-treated WKY but not in SHRSP rats. Platelet 
      reactivity in vitro was measured in native blood by a shear-induced haemostasis 
      test (haemostatometry). Indexes of this test (H1/H2), which inversely correlated 
      with platelet reactivity, were significantly greater in SHRSP than in WKY rats 
      (SHRSP vs. WKY, H1: 1815+/-192 vs. 763+/-75; H2: 7547+/-723 vs. 3536+/-264). This 
      suggests reduced platelet reactivity in SHRSP compared with WKY rats. Thus, the 
      present findings show increased thrombotic tendency in SHRSP rats in vivo despite 
      reduced platelet reactivity in vitro. To explain this contradiction, we suggest 
      that an increased in vivo thrombotic tendency may be due to impaired nitric oxide 
      (NO) release from endothelial cells in SHRSP rats, and that a reduced platelet 
      reactivity in vitro may be due to an adaptation of SHRSP rats to survive at 
      extremely high blood pressure.
FAU - Yamashita, T
AU  - Yamashita T
AD  - Laboratory of Physiology, Faculty of Nutrition and High Technology Research 
      Centre, Kobegakuin University, Arise 518, Igawadani-cho, Kobe 651-2180, Japan. 
      tsutomu@nutr.kobegakuin.ac.jp
FAU - Taka, T
AU  - Taka T
FAU - Nojima, R
AU  - Nojima R
FAU - Ohta, Y
AU  - Ohta Y
FAU - Seki, J
AU  - Seki J
FAU - Yamamoto, J
AU  - Yamamoto J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/administration & dosage/pharmacology
MH  - Adaptation, Physiological
MH  - Animals
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/*metabolism
MH  - Hypertension/etiology
MH  - Lasers
MH  - Male
MH  - Nitric Oxide/*metabolism/pharmacology
MH  - Platelet Activation
MH  - Rats
MH  - Rats, Inbred SHR/*physiology
MH  - Rats, Inbred WKY
MH  - Stress, Mechanical
MH  - Stroke/*etiology
MH  - Thrombosis/etiology
EDAT- 2002/07/02 10:00
MHDA- 2003/03/05 04:00
CRDT- 2002/07/02 10:00
PHST- 2002/07/02 10:00 [pubmed]
PHST- 2003/03/05 04:00 [medline]
PHST- 2002/07/02 10:00 [entrez]
AID - S0049384802000695 [pii]
AID - 10.1016/s0049-3848(02)00069-5 [doi]
PST - ppublish
SO  - Thromb Res. 2002 Mar 15;105(6):507-11. doi: 10.1016/s0049-3848(02)00069-5.