PMID- 12091083
OWN - NLM
STAT- MEDLINE
DCOM- 20030212
LR  - 20191106
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 25 Suppl 1
DP  - 2002 Jul
TI  - Detection of herpesvirus genomes by polymerase chain reaction in cerebrospinal 
      fluid and clinical findings.
PG  - S59-70
AB  - BACKGROUND: The viruses of the Herpesviridae family, in particular herpes simplex 
      virus types 1 (HSV-1) and 2 (HSV-2), cytomegalovirus (CMV), Epstein-Barr virus 
      (EBV), varicella zoster virus (VZV), and human herpesvirus 6 (HHV-6), are 
      responsible for numerous infections of the central nervous system (CNS). These 
      infections manifest as diverse clinical signs, many of which are not specific. 
      The diagnosis of these infections is necessary to make it possible to adapt 
      treatment appropriately, as treatment is specific for the particular virus 
      concerned. OBJECTIVES: To apply a polymerase chain reaction (PCR) technique for 
      the diagnosis in a single reaction of the six herpesviruses most frequently 
      detected in the cerebrospinal fluid (CSF) and to analyse clinical events in 
      patients presenting positive results in PCR for herpesviruses. STUDY DESIGN: We 
      studied 141 patients, from whom 180 CSF samples were collected. The clinical 
      files of the patients were consulted retrospectively, and a list of clinical 
      signs was recorded. After testing by targeted PCR, at the clinician's demand, we 
      tested these samples by herpes consensus PCR, which detects six herpesviruses 
      (HSV-1, HSV-2, CMV, EBV, VZV, HHV-6), in a single PCR. RESULTS: Targeted PCR 
      tests identified 25 CSF samples (13.9%), corresponding to 18 patients (12%), as 
      positive. The herpes consensus PCR test detected 49 samples (27.2%) as positive, 
      resulting in the identification of 54 individual viruses (four samples displayed 
      co-infection) from 39 patients (27%). 130 CSF samples, from 101 patients, tested 
      negative by both techniques. 23 HIV-positive patients (30.6%), three HIV-negative 
      immunocompromised patients (27%), and 14 immunocompetent patients (25%) were CSF 
      PCR-positive. In HIV-positive patients, CMV was the virus most frequently 
      identified (13%), followed by EBV (10.6%), VZV (5.3%) and finally HSV-1 and HSV-2 
      (both 1.3%). We did not detect HHV-6 in any of these samples. We detected only 
      HSV-2, EBV and VZV in the 11 HIV-negative immunocompromised patients. CSF samples 
      of immunocompetent patients contained mostly VZV (9%) and HSV-1 (7.3%). 
      CONCLUSIONS: The herpes consensus PCR for a given virus was more sensitive than 
      the standard, targeted PCR used in our laboratory. The clinical signs presented 
      by patients infected with HSV-1, HSV-2 and CMV were similar to those reported in 
      previous studies. For VZV, we report the possibility of mild, transient cerebral 
      viral reactivation. Our data on the detection of EBV by PCR suggest that the PCR 
      test is of predictive value for cerebral lymphoma in immunocompromised patients. 
      The possible role of HHV-6 in a subacute neurological disorder merits further 
      investigation.
FAU - Minjolle, Sophie
AU  - Minjolle S
AD  - Laboratoire de Bacteriologie-Virologie, Universite Rennes 1, 2 avenue, du Pr Leon 
      Bernard, CS 34317, 35 043 Rennes cedex, France.
FAU - Arvieux, C
AU  - Arvieux C
FAU - Gautier, A L
AU  - Gautier AL
FAU - Jusselin, I
AU  - Jusselin I
FAU - Thomas, R
AU  - Thomas R
FAU - Michelet, C
AU  - Michelet C
FAU - Colimon, Ronald
AU  - Colimon R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (DNA, Viral)
RN  - 0 (Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CD4 Lymphocyte Count
MH  - Cerebrospinal Fluid/virology
MH  - DNA, Viral/analysis
MH  - HIV Infections/immunology/virology
MH  - HIV Seronegativity
MH  - Herpesviridae/genetics/*isolation & purification
MH  - Herpesviridae Infections/cerebrospinal fluid/*diagnosis/virology
MH  - Humans
MH  - Immunocompetence
MH  - Immunocompromised Host
MH  - Leukocyte Count
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Proteins/analysis
EDAT- 2002/07/02 10:00
MHDA- 2003/02/14 04:00
CRDT- 2002/07/02 10:00
PHST- 2002/07/02 10:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/07/02 10:00 [entrez]
AID - S1386653202000355 [pii]
AID - 10.1016/s1386-6532(02)00035-5 [doi]
PST - ppublish
SO  - J Clin Virol. 2002 Jul;25 Suppl 1:S59-70. doi: 10.1016/s1386-6532(02)00035-5.