PMID- 12091423
OWN - NLM
STAT- MEDLINE
DCOM- 20020715
LR  - 20220408
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 43
IP  - 7
DP  - 2002 Jul
TI  - Detection of herpes simplex virus type 1 in human ciliary ganglia.
PG  - 2244-9
AB  - PURPOSE: To determine whether herpes simplex virus type 1 (HSV-1) DNA is present 
      in the ciliary ganglion (CG). METHODS: Fifty CG and 47 trigeminal ganglia (TG) 
      were resected from 63 formalin-fixed cadavers between 56 and 98 years of age that 
      had been embalmed within 12 hours of death. The donors had no known active HSV 
      infection at the time of death. DNA was extracted from all ganglia by 
      proteinase-K digestion (TG) or digestion by a mild lysis buffer (CG). DNA was 
      amplified by polymerase chain reaction for sequences from human chromosome 18, 
      D18S1259 (positive control), and from the HSV-1 DNA polymerase gene, U(L)30. The 
      amplified DNA was separated by agarose gel electrophoresis, transferred to nylon 
      membranes, and hybridized with the appropriate digoxigenin-labeled probe that was 
      detected by alkaline phosphatase-conjugated monoclonal antibody. RESULTS: The 
      D18S1259 sequence was amplified from 47 TG and 30 CG samples. Of these samples, 
      32 (68.0%) of the 47 TG samples and 20 (66.6%) of the 30 CG samples were positive 
      for the UL(30) HSV-1 sequence. CONCLUSIONS: Using amplification of HSV-1 DNA as a 
      surrogate marker of latency, the finding that the frequency of HSV-1 in the CG 
      was approximately the same as that of the TG suggests that the CG may be an 
      additional site of HSV-1 latency in humans. Active infection in or reactivation 
      of HSV-1 from non-TG sites may explain why this virus is able to infect sites, 
      such as the retina, that have no direct connections to the trigeminal nerve.
FAU - Bustos, Daniel E
AU  - Bustos DE
AD  - Department of Cellular and Structural Biology, University of Texas Health Science 
      Center at San Antonio, San Antonio, TX, USA.
FAU - Atherton, Sally S
AU  - Atherton SS
LA  - eng
GR  - EY 06012/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Ciliary Body
MH  - DNA Primers/chemistry
MH  - DNA Probes/chemistry
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Ganglia, Parasympathetic/*virology
MH  - Gene Amplification
MH  - Herpesvirus 1, Human/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Trigeminal Ganglion/virology
MH  - Virus Latency
EDAT- 2002/07/02 10:00
MHDA- 2002/07/16 10:01
CRDT- 2002/07/02 10:00
PHST- 2002/07/02 10:00 [pubmed]
PHST- 2002/07/16 10:01 [medline]
PHST- 2002/07/02 10:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2244-9.