PMID- 12092025
OWN - NLM
STAT- MEDLINE
DCOM- 20021220
LR  - 20091119
IS  - 0024-7758 (Print)
IS  - 0024-7758 (Linking)
VI  - 47
IP  - 6
DP  - 2002 Jun
TI  - FISH analysis of Y chromosome long arm deletions in subfertile men considering 
      ICSI. A report of two cases.
PG  - 515-8
AB  - BACKGROUND: Men with Y chromosome long arm deletions, resulting in infertility, 
      form a very significant group of patients, with a view to treatment. With recent 
      advances in assisted reproductive techniques, it is expected that if these 
      patients undergo intracytoplasmic sperm injection, their male offspring will 
      inherit the same deleted regions of Y chromosomes. Hence, characterization of 
      these deleted Y regions provides information, allowing patients to make informed 
      decisions about reproduction. Fluorescence in situ hybridization (FISH), with 
      probes along the Y chromosome, is very helpful in identification of the exact 
      breakpoints. CASES: Two men with complaints of infertility on examination showed 
      low or no sperm in their semen samples. Routine cytogenetic analysis of the 
      peripheral blood showed a small marker chromosome. This marker was identified as 
      the Y chromosome by FISH. Subsequently, other probes along the Y long arm were 
      used to characterize the extent of the deletion. CONCLUSION: With the use of 
      fluorescence-labelled probes, it was possible to identify the extent of the Y 
      chromosome deletion. The distal Yq11 region had been lost in both patients, 
      resulting in oligospermia and azoospermia. Characterizing markers by FISH gave 
      good guidelines to the patients about the possible effects on offspring, allowing 
      them to make appropriate decisions.
FAU - Gole, Leena A
AU  - Gole LA
AD  - Department of Obstetrics and Gynaecology, Nationl University Hospital, 5 Lower 
      Kent Ridge Road, Singapore 119074.
FAU - Ng, Soon Chye
AU  - Ng SC
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Reprod Med
JT  - The Journal of reproductive medicine
JID - 0173343
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - *Chromosome Deletion
MH  - Cytogenetic Analysis
MH  - Genetic Markers/genetics
MH  - Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Oligospermia/*diagnosis/*genetics/therapy
MH  - Sperm Count
MH  - *Sperm Injections, Intracytoplasmic
MH  - Y Chromosome/*genetics
EDAT- 2002/07/03 10:00
MHDA- 2002/12/21 04:00
CRDT- 2002/07/03 10:00
PHST- 2002/07/03 10:00 [pubmed]
PHST- 2002/12/21 04:00 [medline]
PHST- 2002/07/03 10:00 [entrez]
PST - ppublish
SO  - J Reprod Med. 2002 Jun;47(6):515-8.